Abstract：OBJECTIVE To compare the limits of detection (LODs) of TLC obtained with three evaluation approaches in Chinese Pharmacopoeia 2015, and facilitate selection of LOD evaluation approach in TLC method validation. METHODS After development, the spots of strychnine, berberine hydrochloride, and ten chemical dyes were first detected by human eyes and then scanned by thin layer scanner to obtain the LODs based on visual evaluation, signal-to-noise, and standard deviation of the response and the slope. RESULTS The LODs obtained by the three approaches showed no significant difference. For strychnine and 10 chemical dyes,the LODs based on the three approaches were close to each other. For berberine hydrochloride, the LOD obtained by visual evaluation was slightly lower than those obtained by the other two approaches. CONCLUSION All the three approaches are feasible to determine LOD in TLC. The approach based on visual evaluation is rapid and reliable, which might be the first choice for LOD determination in TLC.
何风艳, 李静, 何轶, 郑笑为, 戴忠, 马双成. 3种检测限测定方法在薄层色谱中的应用比较[J]. 中国药学杂志, 2019, 54(18): 1515-1519.
HE Feng-yan, LI Jing, HE Yi, ZHENG Xiao-wei, DAI Zhong, MA Shuang-cheng. Comparison of Three LOD Determination Approaches in TLC. Chinese Pharmaceutical Journal, 2019, 54(18): 1515-1519.
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