两种白茅根聚合酶链式反应法-限制性片段长度多态性分析鉴别方法的研究

朱晓燕, 黄韵璇, 黄昌杰, 蓝永锋, 侯惠婵, 陈伟盛

中国药学杂志 ›› 2019, Vol. 54 ›› Issue (18) : 1486-1490.

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中国药学杂志
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中国药学杂志 ›› 2019, Vol. 54 ›› Issue (18) : 1486-1490. DOI: 10.11669/cpj.2019.18.006
·论著·

两种白茅根聚合酶链式反应法-限制性片段长度多态性分析鉴别方法的研究

  • 朱晓燕1, 黄韵璇1, 黄昌杰2, 蓝永锋3, 侯惠婵1, 陈伟盛1*
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Identification of Two Imperatae Rhizoma Based on PCR-RFLP

  • ZHU Xiao-yan1, HUANG Yun-xuan1, HUANG Chang-jie2, LAN Yong-feng3, HOU Hui-chan1, CHEN Wei-sheng1*
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摘要

目的 从分子水平鉴别2种不同性状的白茅根。方法 采用植物通用DNA条形码ITS1、ITS2、psbA-trnH、rbcL和matK对30份白茅根样品的序列进行比较研究,分析2种不同性状白茅根的多个位点差异。结果 30批白茅根的psbA-trnH、rbcL和matK基因序列无位点差异,ITS1序列含3个关键差异位点,ITS2序列含1个关键差异位点,且ITS2序列上的关键差异位点可被限制性内切酶HpyCH4Ⅲ识别。结论 ITS1和ITS2序列可作为2种不同性状白茅根鉴别用的DNA条形码序列,且利用ITS2序列上可被限制性内切酶HpyCH4Ⅲ识别的关键差异位点可建立2种不同性状白茅根的PCR-PFLP鉴别方法。

Abstract

OBJECTIVE To identify two different characters of Imperatae Rhizoma on a molecular level. METHODS A total of 30 samples of Imperatae Rhizoma were analyzed using DNA barcoding method based on ITS1, ITS2, psbA-trnH, matK, and rbcL genes. The DNA sequences were further used for site difference analysis. RESULTS There is no site difference on psbA-trnH, matK, rbcL of two different characters of Imperatae Rhizoma, three key site differences on ITS1, and one key site difference on ITS2. The key site difference on ITS2 among two different characters of Imperatae Rhizoma can be recognized by the restriction enzyme HpyCH4Ⅲ. CONCLUSION ITS2/ITS1 regions can accurately and efficiently identify two different characters of Imperatae Rhizoma, and PCR-RFLP analysis of the ITS2 region with HpyCH4Ⅲ is a method to distinguish two different characters of Imperatae Rhizoma.

关键词

白茅根 / DNA条形码 / 位点差异 / 聚合酶链式反应-限制性片段长度多态性

Key words

imperatae rhizoma / DNA barcoding / site difference / PCR-PFLP

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朱晓燕, 黄韵璇, 黄昌杰, 蓝永锋, 侯惠婵, 陈伟盛. 两种白茅根聚合酶链式反应法-限制性片段长度多态性分析鉴别方法的研究[J]. 中国药学杂志, 2019, 54(18): 1486-1490 https://doi.org/10.11669/cpj.2019.18.006
ZHU Xiao-yan, HUANG Yun-xuan, HUANG Chang-jie, LAN Yong-feng, HOU Hui-chan, CHEN Wei-sheng. Identification of Two Imperatae Rhizoma Based on PCR-RFLP[J]. Chinese Pharmaceutical Journal, 2019, 54(18): 1486-1490 https://doi.org/10.11669/cpj.2019.18.006
中图分类号： R969   

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