Abstract：OBJECTIVE To explore the feasibility of using colloidal gold immunochromatography for quantitative detection of aflatoxin B1 in traditional Chinese medicine. METHODS Negative samples were used to investigate matrix interference by different levels of spikes.The rapid inspection performance was evaluated by examining the precision, sensitivity, linearity, repeatability and recovery rate. The sample was determined by rapid test method and verified by HPLC. RESULTS High-concentration and low-concentration aflatoxin B1 reference materials were added to the negative sample matrix. After the measurement, it was found that there were matrix interferences in the samples such as tangerine peel and cassia seed, and the interference was greater when the concentration was increased. So high dilution factor was used to reduce the interference. The precision RSD of the rapid test method was 4.6% (n=10), the reproducibility RSD was 4.1% (n=6), and the recoveries of different samples were between 72.8% and 112.8%. The overall performance of the method was good. A total of 43 batches of 19 kinds of medicinal materials such as silkworm, cockroach and leeches were detected by two methods. The coincidence rate between the fast test and the HPLC test was 83.7%. Therefore, the results obtained by the two detection methods were considered to be approximate. CONCLUSION Colloidal gold immunochromatographic rapid test method can be used for the quantitative detection of aflatoxin B1 in some traditional Chinese medicines, and provides technical support for the establishment and improvement of relevant rapid detection standard methods.
李耀磊, 刘丽娜, 姚云, 赵小旭, 周霞, 金红宇, 马双成. 基于胶体金免疫层析技术对中药材中黄曲霉毒素B1的定量检测研究[J]. 中国药学杂志, 2019, 54(17): 1432-1437.
LI Yao-lei, LIU Li-na, YAO Yun, ZHAO Xiao-xu, ZHOU Xia, JIN Hong-yu, MA Shuang-cheng. Quantitative Detection of Aflatoxin B1 in Traditional Chinese Medicine by Colloidal Gold Immunochromatography. Chinese Pharmaceutical Journal, 2019, 54(17): 1432-1437.
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2019, Vol. 54 
