Abstract：OBJECTIVE To conduct a comprehensive and systematic qualitative investigation on chemical components in Shengmai injection. METHODS Gas chromatography-mass spectroscopy (GC-MS) with pre-column derivatization, rapid resolution liquid chromatography-ion trap-tandem mass spectroscopy (RRLC-IT-MSn) and ion exchange chromatography (IEC) with post-column derivatization were applied in identification of saccharides, saponins, lignins and amino acids in the sample. Furthermore, preliminary investigation on test of macromolecules was performed using size exclusion chromatography-evaporative light scattering detection (SEC-ELSD). RESULTS Fructose, glucose, sucrose, maltose, 5-hydroxymethyl-2-furaldehyde, 19 saponins, 14 lignins and 16 amino acids were found in Shengmai injection. CONCLUSION The proposed methods are simple and accurate. The main chemical substantial basis of Shengmai injection is clarified, laying a foundation for further assay of chemical components.
聂黎行, 刘瑞, 李静, 戴忠, 王钢力, 马双成. 生脉注射液化学物质基础研究(Ⅰ)——化学成分的定性分析[J]. 中国药学杂志, 2019, 54(17): 1387-1394.
NIE Li-xing, LIU Rui, LI Jing, DAI Zhong, WANG Gang-li, MA Shuang-cheng. Chemical Substantial Basis of Shengmai Injection(Ⅰ): Qualitative Analysis of Chemical Components. Chinese Pharmaceutical Journal, 2019, 54(17): 1387-1394.
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2019, Vol. 54 
