Abstract：OBJECTIVE To study the metabolic differences of the effective fractions of total glycosides of Jinkui Shenqi Pills in liver homogenate and blood by high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-ESI/MSn). METHODS The in vitro metabolism of the glycosides was studied using rat liver homogenate and the in vivo metabolism was investigated in rats. The metabolites were detected by HPLC-ESI/MS/MS. According to the molecular ion peak in negative ion mode, the molecular weight information of the compound was obtained. RESULTS A total of 13 chemical components were detected in the rat liver homogenate after incubation for 20 min. Eight prototype components were detected in both liver homogenate and plasma while pentagalloylglucose was only found in the liver homogenate. A total of nine metabolites were detected in plasma, and only four metabolites were found in the liver, which were shared by those in plasma. CONCLUSION The four common metabolites are produced through liver metabolism of the total glycosides. The RESULTS of this study have important implications for revealing the metabolic mechanisms of the total glycoside constituents in vitro and in vivo.
杨波, 王昕蕾, 张蒙蒙, 刘博男, 胡扬, 孙向明, 李文兰. 基于高效液相色谱-电喷雾离子阱质谱法技术研究金匮肾气丸总苷体内外代谢的相关性[J]. 中国药学杂志, 2019, 54(14): 1176-1181.
YANG Bo, WANG Xin-lei, ZHANG Meng-meng, LIU Bo-nan, HU Yang, SUN Xiang-ming, LI Wen-lan. Correlation between In Vitro and In Vivo Metabolism of Total Glycosides of Jinkui Shenqi Pills Based on HPLC-ESI/MSn Technique. Chinese Pharmaceutical Journal, 2019, 54(14): 1176-1181.
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2019, Vol. 54 
