1. School of Pharmacy, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550025, China; 2. School of Medical Sciences, University of Sydney, Sydney, NSW 2006, Australia
Abstract��OBJECTIVE To investigate the effect of tryptanthrin on human breast cancer MCF-7 proliferation and MAPK signaling pathway. METHODS The human breast cancer cell MCF-7 was cultured in vitro, different concentrations of 0.1, 0.5, 1, 5, 10, 25, 50, 100 ��mol��L-1 of tryptanthrin and ERK, p38MAPK and JNK pathway inhibitors were used to treat MCF-7 cell for 24, 48 or 72 h. MTT assay was used to detect cell proliferation activity. The expression level of ERK, p-ERK, p38, p-p38, JNK and p-JNK were measured by Western blot method. RESULTS Low, medium and high doses of tryptanthrin significantly inhibited the proliferation of MCF-7 cells. When MCF-7 cells were treated with different concentrations of tryptanthrin for 24, 48, 72 h, the inhibition rate of cell proliferation was correlated with the drug concentration (r=0.904, r=0.793, r=0.770, P<0.05). The 25 ��mol��L-1 of tryptanthrin significantly inhibits the proliferation of MCF-7 cells for 24 h treatment(P<0.01). The cell proliferation activity of tryptanthrin combined with ERK inhibitor group, tryptanthrin combined with p38 MAPK inhibitor group, tryptanthrin combined with JNK inhibitor group was significantly different from that of tryptanthrin group(P<0.01 or P<0.001); MCF-7 cells was treated with 6.25, 12.5, 25 ��mol��L-1 of tryptanthrin, the expression of p-ERK and p-JNK increased significantly(P<0.05 or P<0.01); MCF-7 cells was treated with 25 ��mol��L-1 of tryptanthrin, the expression of p-p38 MAPK increased significantly(P<0.05); the expression of ERK, p38 MAPK and JNK did not change significantly. CONCLUSION Tryptanthrin can inhibit the proliferative activity of MCF-7 cells, and its mechanism may be closely related to the activation of MAPK signaling pathway.
����, ���췼, Donna Lai, Junlae Cho, ������, ���鴺. ɫ��ͪͨ��˿��ԭ�����ø�ź�ͨ·�������ٰ�MCF-7ϸ����ֳ��Ӱ��[J]. �й�ҩѧ��־, 2019, 54(9): 693-698.
ZHOU Wei, ZENG Qing-fang, Donna LAI, Junlae CHO, ZHANG Xiao-yan, SHENG Xiang-chun. Effect of Tryptanthrin on Proliferation of Human Breast Cancer MCF-7 Cells Via MAPK Signaling Pathway. Chinese Pharmaceutical Journal, 2019, 54(9): 693-698.
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2019, Vol. 54 
