目的 探讨DNA条形码结合高分辨熔解曲线(Bar-HRM)技术在红花龙胆及其混伪品鉴定中的应用。方法 基于ITS2条形码,对红花龙胆及4种混伪品的序列进行扩增并分析高分辨熔解曲线。结果 利用ITS2序列,Bar-HRM技术能有效区分红花龙胆及其混伪品,Bar-HRM结果与专家性状和DNA条形码结果一致。结论 本实验表明,Bar-HRM技术适用于红花龙胆及其混伪品的快速鉴定,可作为DNA条形码技术的有效补充,在中药的快速鉴定领域具有广阔的应用前景。
Abstract
OBJECTIVE To apply DNA barcoding coupled with high resolution melting analysis to distinguish Gentiana rhodantha from its adulterants. METHODS The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. RESULTS The ITS2 molecular regions coupled with HRM analysis can effectively differentiate five herbal species, including two Gentiana rhodantha and their four common adulterants. CONCLUSION DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which may contribute to the quality control of traditional Chinese medicine in the natural health product industry.
关键词
DNA条形码 /
高分辨熔解曲线 /
红花龙胆 /
混伪品 /
鉴定 /
民族药
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Key words
DNA barcoding /
high resolution melting /
Gentiana rhodantha /
adulterant /
identification /
ethnodrug
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中图分类号：
R927
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参考文献
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