��ҽ�ƴ�ѧ������������̳ҽԺ, a. ҩ����; b. ��Ѫ��, c. �ٴ�������ҩ����������ѧ���۱������ص�ʵ����, d. �ٴ�������ҩ����������ѧ���۹��ʺ�������ʵ����, ���� 100038
Rapid Determination of Teicoplanin Concentration in Human Plasma by UPLC and Its Application
SHI Zheng-yuana,c,d, ZHUANG Jian-meib, GU Hong-yana,c,d, YANG Xin-yua,c,d, YAN Dana,c,d*
a. Department of Pharmacy; b. Department of Blood Transfusion; c. Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use; d. Joint Laboratory for International Cooperation of Bio-characteristic Profiling for Evaluation of Clinical Rational Drug Use Evaluation, Beijing Shijitan Hospital Affiliated to Capital University of Medical Sciences, Beijing 100038, China
Abstract��OBJECTIVE To establish a rapid ultra-high performance liquid chromatography method for determining teicoplanin concentration in human plasma. METHODS The samples were chromatographed on Waters Acquity UPLC BEH-C18 column using gradient elution method(mobile phase A:20 mmol��L-1 ammonium acetate;mobile phase B:acetonitrile). The absorption wavelength was set at 215 nm. The column temperature were maintained at 35 ��. The teicoplanin concentration was calculated by internal standard method and external standard method, respectively. The results of the two methods were compared by paired t-test using SPSS statisticals software. RESULTS The liner range of the calibration curve for teicoplanin was 3.15-100 ��g��mL-1(r=0.999 9). The concentrations calculated by the two methods had good correlation without significant difference. CONCLUSION This method is proved to be rapid, sensitive and suitable for the therapeutic drug monitoring and pharmacokinetic investigation of teicoplanin in human plasma.
ʱ����, ׯ����, �˺���, �����, ۳��. ����ЧҺ��ɫ�����ٲⶨ��Ѫ�����濼������Ũ�ȼ���Ӧ��[J]. �й�ҩѧ��־, 2019, 54(6): 484-488.
SHI Zheng-yuan , ZHUANG Jian-mei , GU Hong-yan , YANG Xin-yu , YAN Dan. Rapid Determination of Teicoplanin Concentration in Human Plasma by UPLC and Its Application. Chinese Pharmaceutical Journal, 2019, 54(6): 484-488.
JIANG H Y, CAO W, CHEN Y G, et al. Clinical effects of teicoplanin and vancomycin on treatment of gram-positive bacteria infections. Chin J Nosocomiol(�л�ҽԺ��Ⱦѧ��־),2018,28(2): 173-176.
[2]
TOBIN C M,LOVERING A M,SWEENEY E,et al.Analyses of teicoplanin concentrations from 1994 to 2006 from a UK assay service. J Antimicrob Chemother,2010,65(10): 2155-2157.
[3]
WANG T T,LI N A,HU S S, et al. Factors on trough teicoplanin levels, associations between levels, efficacy and safety in patients with gram-positive infections. Int J Clin Pharmacol Ther,2015,53( 5): 356-362.
[4]
JIANG H Y,CAO W,CHEN Y G, et al. Determination of teicoplanin in serum by HPLC.J Guangdong Pharm Univ(�㶫ҩѧԺѧ��),2011,27(5): 496-498.
[5]
HE M,LIU J,ZHOU L J. Determination of teicoplanin in human serum by RP-HPLC and its application.Chin Hosp Pharm J(�й�ҽԺҩѧ��־), 2017,37(12): 1163-1166.
[6]
BEGOU O,KOUTOU A,RAIKOSN,et al. An ultra-high pressure liquid chromatography-tandem mass spectrometry method for the quantification of teicoplanin in plasma of neonates.J Chromatogr B,2017,1047 :215-222.
[7]
CHEN J,WANG G L,YAO L W,et al. Recent application of ultra performance liquid chromatography (UPLC) in pharmaceutical analysis. Chin J Pharm Anal(ҩ�������־),2008,28(11): 1976-1981.
[8]
MATTHEWS P C,CHUE A L,WYLLIE D, et al. Increased teicoplanin dosesare associated with improved serum levels but not drug toxicity.J Infect, 2014,68(1): 43-49.
[9]
LI P M,LIU X,LIU J Y,et al. Rapid determination of meropenem concentration in human plasma by column switching ultrahigh performance liquid chromatography. Chin Pharm J (�й�ҩѧ��־),2014,49(9): 776-780.
[10]
FUNG F H, TANG J C, HOPKINS J P, et al. Measurement of teicoplanin by liquid chromatography-tandem mass spectrometry:development of a novel method. Ann Clin Biochem,2012,49(Pt5):475-481.
[11]
HU S S, PANG C S, DONG H Y, et al. Determination of teicoplanin serum concentration in critically ill patients. Chin Hosp Pharm J (�й�ҽԺҩѧ��־),2011,31(9):753-755.
2019, Vol. 54 
