1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; 2. China Medical University, Shenyang 110001,China; 3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Abstract��OBJECTIVE To explore the anti-osteoporotic mechanism of icariin based on osteoblast and osteoclast proteomics. METHODS The cell proliferation was determined by MTT assay. The activity of alkaline phosphatase (ALP) in osteoblasts and tartrate-resistant acid phosphatase (TRAP) in osteoclast were measured using ELISA kit. Alizarin red staining was used to observe the formation of bone mineralized nodules in osteoblasts. The number and area of bone resorption pit formed on bone slices by osteoclasts were used to characterize the activity of osteoclastic bone resorption. Two-dimensional gel electrophoresis was used to observe the changes of icariin-treated osteoblast and osteoclast proteomics. TOF/MS/MS analysis was applied to identify the differentially expressed proteins. RESULTS Icariin significantly enhanced the osteoblastic bone formation and inhibited the osteoclastic bone resorption, and up-regulated or down-regulated expression of nine proteins in osteoblast and osteoclast, respectively. CONCLUSION Icariin regulates bone metabolism through involving multiple biological process, including energy metabolism, oxidative stress, purine synthesis and cell growth and differentiation.
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2019, Vol. 54 
