Abstract��In order to identify the endophytic fungus MD76 and isolate its secondary metabolite. METHODS The identification of strain MD76 is based on morphological characteristics and sequence alignment analysis of 18s rDNA ITS, the secondary metabolites were isolated by chromatography technology; and their structures were elucidated by extensive spectroscopic analysis. RESULTS The strain MD76 was identified as Phyllosticta capitalensis; and seven compounds were gained, including benzoic acid(1), 4-hydroxybenzoic acid(2), 4-hydroxybenzene propanoic acid(3), paeoniflorin(4), oxypaeoniflorin(5), gallic acid(6), apigenin(7). CONCLUSION The endophytic fungus P. capitalensis was gained firstly from Paeonia suffruticosa and seven compounds were isolated from P. capitalensis for the first time.
GULHANE P A, GOMASHE A V, PATNE M K. Endophytic fungi: a source of novel enzymic antioxidants and biologically active secondary metabolites[J]. Int J Recent Sci Res, 2016,(1):8226-8231.
[2]
STIERLE A, STROBEL G, STIERLE D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew[J]. Science, 1993, 260(5105): 214-216.
[3]
YAN H J, ZHAO W, GENG Y L, et al. Chemical constituents from the flower petals of Paeonia suffruticosa Andr[J]. Nat Prod Res Dev(��Ȼ�����о��뿪��), 2015,27(12):2056-2059.
[4]
WEI J C. Handbook of Fungal Identification(��������ֲ�)[M]. Shanghai: Shanghai Science Technology Press, 1979.
[5]
WEISING K, NYBOM H, WOLFF K. DNA Finger-Printing in Plants and Fungi[M]. Florida: CRC Press, 1995: 1-322.
[6]
ZHAO X L, NIU Y C, DENG H. A survey of pathogenic fungi on five common gramineous weeds in Henan Province[J]. Plant Protect(ֲ�ﱣ��), 2013, 39(1):128-132.
[7]
ZHANG Y G, LIU S C, LIU H W. Cycloaspeptides F and G, cyclic pentapeptides from a cordyceps-colonizing isolate of Isaria farinosa[J]. J Nat Prod, 2009, 72(7):1364-1367.
[8]
TANG K, LI B, GUO S X. Analysis of diversity of endophytic fungi in Salvia miltiorrhiza from Henan in China[J]. Chin Pharm J(�й�ҩѧ��־), 2015, 50(7):570-573.
[9]
CHEN J, MENG Z X, XING Y M, et al. Isolation and identification of endophytic fungi from five medicinal plants species of Orchidaceae[J]. Chin Pharm J(�й�ҩѧ��־), 2017, 52(4):267-271.
[10]
LIU J F, ZHNG X M, XUE D Q, et al. Studies on chemical constituents from leaves of Isatis indigotica[J]. China J Chin Mater Med(�й���ҩ��־), 2006, 31(23):1961-1965.
[11]
WANG X J, XIE X, LUO X, et al. Chemical constituents from Rhodiola wallichiana var. cholaensis(I)[J]. Chin Tradit Herb Drugs(�в�ҩ), 2015, 46(23):3471-3474.
[12]
SHI L L, MA G X, YANG J S, et al. Chemical constituents from plant of Stelleropsis tianschanica[J]. Chin Tradit Herb Drugs(�в�ҩ), 2016, 47(2):223-226.
[13]
LIN H C, DING H Y, WU T S, et al. Monoterpene glycosides from Paeonia suffruticosa[J]. Phytochemistry, 1996, 41(1):237-242.
[14]
LIU P, LI L, DENG R X, et al. Monoterpene glucosides in seed cakes of Paeonia ostii[J]. Chin Pharm J(�й�ҩѧ��־), 2013, 48(17):1253-1256.
[15]
HU H Y, YANG Y, YU N J, et al. Study on chemical constituents of bark of Paeonia suffruticosa[J]. China J Chin Mater Med(�й���ҩ��־), 2006, 31(21):1793-1794.
[16]
HUO L N, WANG W, LIU Y, et al. Chemical constituents from leaves of Perilla frutescens[J]. Chin Tradit Herb Drugs(�в�ҩ), 2016, 47(1):26-31.
[17]
GUO R B, WANG G F, ZHAO A P, et al. Paeoniflorin protects against ischemia-induced brain damages in rats via inhibiting MAPKs/NF-��B-mediated inflammatory responses[J]. PLoS One, 2012,7(11):e49701.
[18]
MAO Q Q, ZHONG X M, FENG C R, et al. Protective effects of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via antioxidant mechanisms and Ca2+ antagonism[J]. Cell Mol Neurobiol, 2010, 30(7):1059-1066.
2018, Vol. 53 
