目的 对一株具有抗菌活性的牡丹内生真菌MD76进行鉴定并对其次级代谢产物进行分离。方法 采用形态学观察结合5.8s rDNA ITS序列分析鉴定菌株MD76的种类;利用硅胶、凝胶柱层析等技术对该菌的大米发酵产物乙酸乙酯浸提物进行分离,并通过波谱数据对分离化合物鉴定。结果 鉴定菌株MD76为首都叶点霉Phyllosticta capitalensis,并从该菌发酵物中分离到7种化合物:苯甲酸(1)、对羟基苯甲酸(2)、对羟基苯丙酸(3)、芍药苷(4)、氧化芍药苷(5)、没食子酸(6)、芹菜素(7)。结论 首都叶点霉P. capitalensis为首次从牡丹健康组织中分离获得,7种化合物均为首次从该种真菌中提取鉴定。
Abstract
In order to identify the endophytic fungus MD76 and isolate its secondary metabolite. METHODS The identification of strain MD76 is based on morphological characteristics and sequence alignment analysis of 18s rDNA ITS, the secondary metabolites were isolated by chromatography technology; and their structures were elucidated by extensive spectroscopic analysis. RESULTS The strain MD76 was identified as Phyllosticta capitalensis; and seven compounds were gained, including benzoic acid(1), 4-hydroxybenzoic acid(2), 4-hydroxybenzene propanoic acid(3), paeoniflorin(4), oxypaeoniflorin(5), gallic acid(6), apigenin(7). CONCLUSION The endophytic fungus P. capitalensis was gained firstly from Paeonia suffruticosa and seven compounds were isolated from P. capitalensis for the first time.
关键词
牡丹 /
首都叶点霉 /
内生真菌 /
次级代谢产物 /
单萜苷
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Key words
Paeonia suffruticosa /
Phyllosticta capitalensis /
endophytic fungi /
secondary metabolites /
monoterpene glucosides
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中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金项目资助(U1404829);河南省高等学校重点科研项目资助(18A180013,18A210009);河南省自然科学基金(182300410075)
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