Chemical Constituents of Ethyl Acetate Fraction from Dioscorea bulbifera
WANG Guo-kai, ZHENG Juan, YU Yang, ZHANG Nan, LIU Hui-wen, SUN Yun-peng, LIU Jin-song*
Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
Abstract��OBJECTIVE To study the chemical constituents of ethyl acetate fraction from Dioscorea bulbifera. METHODS This compounds were isolated and purified with silica gel and Sephadex LH-20 and preparative liquid chromatography. Their structures were determined on the basis of physicochemical properties and spectroscopic analysis. RESULTS Twenty-four compounds were isolated and identified as ephemeranyhoquinone(1), N-methyl-2-pyrolidinone(2), aurantiamide acetate(3), 3,4-dihydroxybenzoic acid ethyl ester(4), 2, 7-dihydroxy-3, 4-dimethoxyphenanthrene(5), 4, 5-dihydroblumenol A(6), C-veratroylglycol(7), teasperol(8), vomifoliol(9), Z-6, 7-ligustilide(10), 2, 3, 7-trihydroxy-4-methoxyphenanthrene(11), p-hydroxyphenylacetic acid methyl ester(12), 3, 5, 7, 4��-tetrahydroxyflavone(13), 3, 5, 3��-trimethoxyquercetin(14), 3, 4-dihydroxybenzoic acid(15), 3, 5, 7, 3��, 4��-pentahydroxyflavone(16), daucosterol(17), 5, 3 ��, 4��-trihydroxy-3, 7-dimethoxyflavone(18), epicatechin(19), 3, 7-dihydroxy-2, 4-dimethoxy-9, 10-dihydrophenanthrene(20), methyl eucomate(21), catechins(22), 5, 7-dihydroxy-3, 4��-dimethoxyflavone(23), and 3, 4-dihydroxybenzoic acid methyl ester(24). CONCLUSION Except compounds 11, 14, 15, 18-20, 22 other compounds are isolated from the plant for the first time.
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2018, Vol. 53 
