Abstract��OBJECTIVE To observe the inhibition of saposhnikovan sulphate obtained by ultrasonic extraction(S-USPS) on human leukemia MCF-7 cells in vitro and the effect of S-USPS on the recruitment and re-education of macrophages by MCF-7 cells. METHODS S-USPS was obtained from saposknikovan(SPS) by the method of chlorosulfonic acid-pyridine complex. The inhibition of S-USPS and USPS on proliferation of MCF-7 cells was measured by MTT. THP-1-derived macrophages were differentiated in vitro. The effect of S-USPS on the recruitment of macrophages by MCF-7 cells was detected by transwell migration assay. The effects of S-USPS on the expression and secretion of chemokine were detected by qRT-PCR and ELISA. The effects of S-USPS and CCL3 on the expression of inflammatory cytokines were detected by qRT-PCR. RESULTS The inhibitory rate of S-USPS on the proliferation of MCF-7 was dose-dependent and time-dependent and was higher than that of USPS(P<0.05).S-USPS increased the transcription and secretion of CCL3 by MCF-7 cells. There were higher expressions of IL-1��, IL-6, and TNF-�� mRNA and lower expressions of TGF-��, IL-10 mRNA of macrophages in the S-USPS group than in the control group and MCF-7 supernatant group(P<0.05). The promotion of S-USPS on the expressions of IL-1�� and TNF-�� was inhibited by CCL3 neutralizing antibody(P<0.05). CONCLUSION There are significant evidences that the saposhnikovan sulphate effectively promotes the recruitment of macrophages by MCF-7 cells in vitro, and re-educates their secretion profile toward M1-type.
�ֻ�, ������, ������. ���������������ͨ��CCL3Ӱ�����ٰ�ϸ��MCF-7�Ծ���ϸ����ļ����ѱ������[J]. �й�ҩѧ��־, 2018, 53(20): 1755-1761.
LIN Hua, CAO Ya-li, DING Jing-xuan. Effect of Saposhnikovan Sulphate on the Recruitment and Re-education of Macrophages by MCF-7 Cells In Vitro through CCL3. Chinese Pharmaceutical Journal, 2018, 53(20): 1755-1761.
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2018, Vol. 53 
