目的 观察野生冬虫夏草和冬虫夏草人工繁育品免疫调节作用和抗衰老作用。方法 建立环磷酰胺诱导的免疫抑制小鼠模型,灌胃给予2种冬虫夏草(低、高剂量),观察各组小鼠碳廓清能力、吞噬系数和淋巴细胞转化刺激指数、耳肿胀等指标;建立D-半乳糖所致衰老小鼠模型,灌胃给予2种冬虫夏草(低、高剂量),观察各组小鼠氧化、炎症、脑组织形态等指标。结果 2种冬虫夏草给药组小鼠的免疫调节均表现出一定程度的改善,D-半乳糖诱导的衰老小鼠的抗氧化能力、炎症因子水平、体外NO释放和脑组织形态等病理改变均得到一定程度的改善。结论 野生冬虫夏草和冬虫夏草人工繁育品均具有增强免疫功能和抗衰老作用,具有良好的市场开发运用前景。
Abstract
OBJECTIVE To observe the immune enhancement and anti-aging activities of cultivated and wild Cordyceps sinensis. METHODS Mice were randomly divided into seven groups: high dose cultivated Cordyceps sinensis group, low dose cultivated Cordyceps sinensis group, high dose wild Cordyceps sinensis group, low dose wild Cordyceps sinensis group, control group, model group and positive drug group. Immunosuppression mouse model were induced by cyclophosphamide and the physical status, carbon clearance capacity, phagocytosis coefficient and lymphocyte transformation stimulation index, and ear swelling of mice were observed. The aging mice model was established by subcutaneously injecting D-galactose, and brain histology, antioxidant and anti-inflammatory capacity of mice were observed. RESULTS Both cultivated and wild Cordyceps sinensis increased the body weight and immune organ index, and enhanced the immunity of the mice. Furthermore, both cultivated and wild Cordyceps sinensis ameliorated the pathological changes in brain histology, and enhanced the antioxidant and anti-inflammatory ability of the mice. There was no obvious difference in the immune enhancement and anti-aging activities between cultivated and wild Cordyceps sinensis. CONCLUSION Both cultivated and wild Cordyceps sinensis can improve immunity and retard the aging process. Cultivated Cordyceps sinensis can be used as the alternative choice of wild Cordyceps sinensis in clinic.
关键词
冬虫夏草 /
免疫抑制 /
衰老
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Key words
cultivated and wild Cordyceps sinensis /
immunoenhancement /
anti-aging
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中图分类号：
R285
R963
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参考文献
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脚注
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基金
国家中医药管理局中药饮片质量保障体系研究项目资助(201507002)
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