Abstract��OBJECTIVE To study the bioactivity and chemical constituents of different polar parts from blueberry leaves. METHODS Blueberry leaves were extracted by ethanol and then the extract was sequentially partitioned into five fractions. Silicagel and Sephadex LH-20 chromatographic methods were applied to isolate and purify compounds. Their structures were elucidated by physiochemical properties and spectral analysis.The DPPH�� radical scavenging activity, ��-glycosidase and pancreatic lipase inhibition activity of different polar parts and partial compounds were determined. RESULTS The n-butyl alcohol fraction(BF) showed the highest DPPH�� radical scavenging activity and ��-glycosidase inhibition activity. The ethyl acetate fraction(EAF) showed the strongest pancreatic lipase inhibition activity. A total of five compounds were isolated from the EAF, and their structures were identified as ��-sitosterol(1), quercetin-3-O-��-L-arabinofuranoside(2), quercetin(3), quercetin-3-O-��-D-glucopyranoside(4) and 1-O-caffeoylquinic acid(5). A total of two compounds were isolated from the BF, and their structures were identified as quercetin-3-O-��-L-arabinoside(6) and quercetin-3-O-��-D-glucuronide(7). The results showed that compounds 3 and 5 had very good DPPH�� radical scavenging and pancreatic lipase inhibitory activity, and compounds 1 and 3 had good ��-glucosidase inhibitory activity. CONCLUSION The different polar parts and compounds of blueberry leaves show strong DPPH�� radical scavenging activity, ��-glycosidase and pancreatic lipase inhibition activity. Compounds 1, 2, 5 and 6 are isolated from blueberry leaves for the first time.
����֦, ������, �����, ��, ����ɺ. ��ݮҶ��ͬ���Բ�λ������Լ���ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2018, 53(20): 1729-1733.
WANG Li-zhi, JIANG Zhen-yu, GE Yu-fei, CHEN Zhuo, XU Li-shan. Bioactivity and Chemical Constituents of Different Polar Parts from Blueberry Leaves. Chinese Pharmaceutical Journal, 2018, 53(20): 1729-1733.
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