1. ֣�ݴ�ѧ��һ����ҽԺ, a. ҩѧ��; b. ����ʡ���ٴ�ҩѧ�ص�ʵ����; c. ������,֣�� 450052; 2. ��������ѧҽѧԺ,���� ���� 454000; 3. ��Ĭ�������Ƽ�(�й�)����˾ɫ�����ײ�,�Ϻ� 201206
The Chemical Constituents Study of Naoxintong Capsules Based on UHPLC-Q-Orbitrap HRMS
SUN Zhi1a,b, ZHOU Lin1a,b, LI Zhuo-lun1a,b, SHI Ying-ying1a,b, LIU Li-wei1a,b, ZHOU Pei-pei1a,b, ZHANG Jun1a,b, WANG Xiao-hui1c, WANG Song1a,b, YANG Jie1a,b, NIU Chao2, HOU Peng-yi3, BU Xin-miao2, ZHANG Xiao-jian1a,b*
1a. Department of Pharmacy; 1b. Henan Key Laboratory of Precision Clinical Pharmacy; 1c. Department of Ultrasonography, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 2. Medical college, Henan Polytechnic University, Jiaozuo 454000, China; 3. Chromatography and Mass Spectrometry Division, Thermo Fisher Scientific, Shanghai 201206, China
Abstract��OBJECTIVE To establish a novel ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method to study the chemical composition of Naoxintong capsules rapidly and accurately. METHODS The information of accurate mass in full-MS and multistage fragment ions was obtained by the novel UHPLC-Q-Orbitrap technology. Chemical constituents were identified by comparing the relative retention time and the mass data of the reference substances, meanwhile consulting the reference literature or the Mass Bank, Chemical Book network database. RESULTS Forty-four compounds were finally identified in this study, mainly including flavones, authraquinones, terpenoids and organic acids. CONCLUSION A method is established in this study to identify various chemical constituents of Naoxintong capsules rapidly, accurately and systematically. What's more, our research will lay a sound theoretical foundation and propose a scientific study idea for the quality control improvement, bioactive components recognition and further clinical application of this herbal formulation.
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SUN Zhi, ZHOU Lin, LI Zhuo-lun, SHI Ying-ying, LIU Li-wei, ZHOU Pei-pei, ZHANG Jun, WANG Xiao-hui, WANG Song, YANG Jie, NIU Chao, HOU Peng-yi, BU Xin-miao, ZHANG Xiao-jian. The Chemical Constituents Study of Naoxintong Capsules Based on UHPLC-Q-Orbitrap HRMS. Chinese Pharmaceutical Journal, 2018, 53(19): 1687-1695.
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2018, Vol. 53 
