Abstract��OBJECTIVE To identify seven species from Umbilicariales and study the chemical constituents, antioxidant and anti-inflammatory activities of crude products from the hot-water extraction. METHODS The seven species from Umbilicariales were identified using ITS sequence analysis, and the seven crude polysaccharides were extracted from the species by water extraction method. RESULTSUmbilicaria esculenta, Umbilicaria yunnana, Dermatocarpon miniatum and Lasallia papulosa were identified, and the main components of crude polysaccharides from the species were carbohydrates. The crude polysaccharides (JUY) from Umbilicaria yunnana of Jiangxi had the best antioxidant ability, it exhibited a higher scavenging activity of superoxide radicals (EC50=0.56 mg��mL-1) than Vc (EC50=1.67 mg��mL-1), and the scavenging activity for hydroxyl radicals (EC50=0.53 mg��mL-1) was comparable to Vc (EC50=0.51 mg��mL-1). Anti-inflammatory experimental results show that JUY has the best anti-inflammatory effects, and the better inhibitory effect of IL-1�� than dexamethasone (P<0.01). CONCLUSION The species from Umbilicariales of seven different areas were identified as four genera. The crude polysaccharides (JUY) from Umbilicaria yunnana of Jiangxi had the best antioxidant and anti-inflammatory activities.
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ZHOU Rong, CAO Sen, LUO Dian-hui. Species Identification of Umbilicariales from Seven Areas and Antioxidant and Anti-inflammatory Activities of Crude Polysaccharides. Chinese Pharmaceutical Journal, 2018, 53(19): 1632-1637.
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