Chemical Constituents from Noni Enzyme and Their Antitumor Activities
LU Yu1,2, JIANG Shi-ping1, SUN Dong-xue2, XIANG Fang-fang3, XIA Xin-yi2, ZHANG Dan-yu2, YANG Jun-shan2, XU Xu-dong2, MA Guo-xu2*, WANG Ping1*
1. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China; 2.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; 3.College of Biology and Environmental Science, Jishou University, Jishou 416000, China
Abstract��OBJECTIVE To study the chemical constituents of Noni enzyme (Morinda citrifolia L.) and their antitumor activities. METHODS Compounds were isolated by various chromatographic techniques, including silica gel, TLC, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by their physicochemical properties and 1H-NMR and 13C-NMR data. The in vitro antitumor activities of the isolated compounds were studied by MTT method. RESULTS Sixteen compounds were isolated from Noni enzyme. They were xylogranatinin(1), pelargonic acid(2), 1,5,15-tri-O-methylmorindol(3), sesquipinsapol B(4), (+)-syringaresinol(5), pinonesinol(6), 3-methylhexahydropyrrolopyrazine-1,4-dione(7), (2S)-3��-hydroxybutan-2-yl-2-hydroxypropanoate (8), 3-(sec-butyl)-6-methylpiperazine-2,5-dione(9), cyclo-(L-Pro-L-Leu)(10), gentisic acid(11), vomifoliol(12), scopoletin(13), 3-(2-hydroxy-4,5-dimethoxyphenyl) propanoic acid(14), medioresinol(15), hydroxychavicol(16). CONCLUSION Compounds 1-10, 12 and 14-16 are isolated from Noni enzyme for the first time. Compound 10 displays the stronger cytotoxicity against HepG2 and HeLa cells with an IC50 value of 23.73, 16.55 ��g��mL-1. Compound 5 had a certain inhibitory activity against HeLa cells with an IC50 value of 47.12 ��g��mL-1.
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LU Yu, JIANG Shi-ping, SUN Dong-xue, XIANG Fang-fang, XIA Xin-yi, ZHANG Dan-yu, YANG Jun-shan, XU Xu-dong, MA Guo-xu, WANG Ping. Chemical Constituents from Noni Enzyme and Their Antitumor Activities. Chinese Pharmaceutical Journal, 2018, 53(18): 1552-1556.
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2018, Vol. 53 
