1. ������ҽҩ��ѧ, a. ��(��)ҩ��Դ�о���; b. ҩ��ֲ���Ŵ������о���,���� 730000; 2.����ʡ��ҩ��������о��ص�ʵ������������,���� 730000
UPLC Fingerprints and Genetic Diversity of Gentiana straminea Maximfile Qilian Mountain Areas of Gansu Province
ZHU Tian-tian1a,1b,2, JIN Ling1a,2*, MA Xiao-hui1a, HUANG De-dong1a, HE Wei-wei1a, LIU Guang-yi2
1a. Research Institute of Chinese (Tibetan) Medicinal Resources; 1b. Research Institute of Medicinal Plant Genetic Breeding, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China; 2.Key Laboratory of Traditional Chinese Medicine Quality and Standard, Gansu Province, Lanzhou 730000, China
Abstract��OBJECTIVE To establish the UPLC fingerprints of G.straminea , and analyze its differences of indications ingredient and genetic diversity. METHODS The main chemical composition of dry root were measured by UPLC. Similarity evaluation system for chromatographic fingerprint of TCM(Version 2004 A) was used to analyze the relative peak area of common peaks from differently populations,and cluster analysis of common peaks was carried out by SPSS19.0. Genomic DNA of dry leaves collected were extracted and amplified by ISSR markers. The genetic diversity and genetic distance were calculated by Popgen 32. RESULTS Twelve Common peaks were selected as the fingerprint peaks from 18 populations of G.straminea,and three groups were divided according to the clustering RESULTS of chemical composition. It had the highest levels that was gentiopicroside content and total content of five kinds of chemical components in Shandan County, Zhangye City.Six primers produced 73 bands, among which 72 were polymorphic bands, the average percentage of polymorphie bands(PPB) was 98.63%; Shannon's information index(��) was 0.366 3;Nei's genetic diversity index(H) was 0.231 6. The genetic differentiation coefficient(Gst) and gene flow(Nm) of wild populations were 0.344 1 and 0.953 0.CONCLUSION UPLC Fingerprint of G.straminea can be used as the quality evaluation system.The genetic diversity among species of G.straminea is at higher level, but the level of genetic diversity within populations is higher than that between populations,and genetic variance mainly existed among populations.
������,����,������,�Ƶö�,��,������. ��������ɽ�����黨��ܴUPLCָ��ͼ�����Ŵ��������о�[J]. �й�ҩѧ��־, 2018, 53(14): 1170-1176.
ZHU Tian-tian, JIN Ling, MA Xiao-hui, HUANG De-dong, HE Wei-wei, LIU Guang-yi. UPLC Fingerprints and Genetic Diversity of Gentiana straminea Maximfile Qilian Mountain Areas of Gansu Province. Chinese Pharmaceutical Journal, 2018, 53(14): 1170-1176.
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2018, Vol. 53 
