Molecular Identification of Polygonum chinensis Using DNA Barcoding
HOU Dian-yun1,2, YANG Meng-meng1,2, LI Jiong3,MA Zhan-qiang1, ZHOU Shuang1,2, ZHAO Xing-li1,2,XU Hua-wei1,2, ZHANG Gai-na1
1. College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China; 2. The Luoyang Engineering Research Center of Breeding and Utilization of Dao-di Herbs, Luoyang 471023, China; 3. Chinese Medicinal Materials Production Technology Service Center of Department of Agriculture of Henan Province, Zhengzhou 450000,China
Abstract��OBJECTIVE To identify Polygonum chinensis and its adulterants by ITS2 sequences. METHODS Total genomic DNA of P. chinensis was extracted using the plant genomic DNA kit. The internal transcribed spacer 2(ITS2) regions were amplified. The variable site of ITS2 regions were analysed through MEGA 6.0 software. The intra-versus inter-specific genetic distances of the ITS2 regions was calculated based on the kimura 2-parameter(K2P) model. Neighbor-Joining phylogenetic trees were constructed using MEGA6.0. RESULTS The intraspecific variation of P. chinensis was small. However, the interspecific variation of P. chinensis and its adulterants was small distinct. The secondary structure of ITS2 of P. chinensis and its adulterants has significant difference. NJ trees can identify P. chinensis and its adulterants. CONCLUSION ITS2 Regions can be used to authenticate P. chinensis and its adulterants which provide new METHODS for the identification of P. chinensis. The standard DNA barcodes of P. chinensis are establishment which would lay the foundation of identification and safety clinical drug application of P. chinensis.
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2018, Vol. 53 
