百合知母汤抗抑郁作用机制的网络药理学研究

王博龙, 刘志强, 陈春林

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (12) : 988-995.

PDF(3198 KB)
PDF(3198 KB)
中国药学杂志 ›› 2018, Vol. 53 ›› Issue (12) : 988-995. DOI: 10.11669/cpj.2018.12.010
·论著·

百合知母汤抗抑郁作用机制的网络药理学研究

  • 王博龙, 刘志强, 陈春林
作者信息 +

Network Pharmacology Study of Anti-Depression Mechanism of Baihe Zhimu Tang

  • WANG Bo-long, LIU Zhi-qiang, CHEN Chun-lin
Author information +
文章历史 +

摘要

目的 利用网络药理学探讨百合知母汤抗抑郁的作用机制。方法 针对已报道百合知母汤中抗抑郁的活性成分,并依据中药系统药理学技术平台(TCMSP)等数据库、反向分子对接服务器(DRAR-CPI)、人类基因组注释数据库(GeneCards)预测和筛选百合知母汤的抗抑郁作用靶点。借助Cytoscape 3.5.1软件构建百合知母汤活性成分-作用靶点网络,借助STRING平台构建靶蛋白互作网络,通过生物学信息注释数据库(DAVID)对靶点GO生物过程及KEGG代谢通路进行分析。结果 百合知母汤筛选出11个活性成分,作用于106个抑郁相关靶点,调控神经营养因子、FoxO、胰岛素、丝裂原活化蛋白激酶(MAPK)、PI3K-Akt、催乳素等代谢通路,参与蛋白水解、蛋白磷酸化、信号转导、RNA调控等主要生物过程。结论 百合知母汤可通过作用于神经、激素等相关的靶点及通路,发挥抗抑郁作用。

Abstract

OBJECTIVE To discuss anti-depression mechanism of baihe zhimu tang using network pharmacology. METHODS The reported antidepressant active ingredients in baihe zhimu tang were used to predict the targets of main active ingredients of baihe zhimu tang relying on traditional Chinese medicine pharmacology system technology platform(TCMSP)database,reverse molecular docking server(DRAR-CPI)and annotation of the human genome database(GeneCards).The Cytoscape 3.5.1 software was used to construct the baihe zhimu tang active ingredients-targets network. The STRING platform was used to construct protein-protein interaction network, and biological information annotation databases(DAVID)was used to analyze metabolic pathways and biological processes of the targets. RESULTS There are total 11 active ingredients and 106 anti-depression related targets in the baihe zhimu tang. The 106 targets were mainly involved in regulation of neurotrophin, FoxO, insulin, MAPK, PI3K-Akt, prolactin and proteolysis, signal transduction, protein phosphorylation and RNA regulation relevant biological processes. CONCLUSION Baihe zhimu tang may play a role in the intervention of depression by acting on the relevant neural, hormonal targets and pathways.

关键词

网络药理学 / 百合知母汤 / 抑郁 / 靶点 / 通路

Key words

network pharmacology / baihe zhimu tang / depression / targets / pathways

引用本文

导出引用
王博龙, 刘志强, 陈春林. 百合知母汤抗抑郁作用机制的网络药理学研究[J]. 中国药学杂志, 2018, 53(12): 988-995 https://doi.org/10.11669/cpj.2018.12.010
WANG Bo-long, LIU Zhi-qiang, CHEN Chun-lin. Network Pharmacology Study of Anti-Depression Mechanism of Baihe Zhimu Tang[J]. Chinese Pharmaceutical Journal, 2018, 53(12): 988-995 https://doi.org/10.11669/cpj.2018.12.010
中图分类号: R965   

参考文献


[1] SCHMITT A B, BAUER M, VOLZ H P, et al. Differential effects of venlafaxine in the treatment of major depressive disorder according to baseline severity[J]. Eur Arch Psychiatry Clin Neurosci, 2009, 259(6):329-339.
[2] ZHU N P, PENG L B, XIONG Y Z, et al. Use and analysis of antidepressant[J]. Chin Pharm J(中国药学杂志), 2008, 43(7):556-558.
[3] LI J, GAO S Y, WANG Z H, et al. The review of baihe zhimu tang in prevention and treatment of neurological and mental disease[J]. J North Pharm(北方药学), 2015, 13(4):284-286.
[4] JIA D, CHEN Y C, CHEN D, et al. Efficacy study of anti-depression of baihe zhimu tang[J]. J North Pharm(北方药学), 2014, 11(8):4-6.
[5] YUAN L, LIU Q, FAN J, et al. Influence of baihe zhimu decoction in levels of key factors of CaM signaling pathyway in hippocampus tissue of rats with depression and its antidepresant mechanism[J]. J Jilin Univ(Med Sci Ed)(吉林大学学报:医学版), 2016, 42(4):704-710.
[6] LIU Q. The cAMP pathway-based study of Treatment effects and mechanism of Baihe Zhimu decoction on depression rats[D]. Hubei:Hubei University of Chinese Medicine, 2016.
[7] ZHAO Q Q, LI X, ZENG H M, et al. A network pharmacology based study of regulation effects of the main active components in Honghua injection on cerebrovascular disease network[J]. Chin Pharm J(中国药学杂志), 2015, 50(16):1402-1407.
[8] YU X C, YANG W, WU B L, et al. Predication of anti-diabetes effects of Corydalis yanhusuo Alkaloids with pharmacological network technology and experimental validation in ICR mice[J]. Chin Pharm J(中国药学杂志), 2014, 49(11):913-918.
[9] YUAN W F, TU M Y, CHEN C, et al. Network pharmacology study of compound Danshen dropping pill based on molecular docking and recognition of biological network function modules[J]. Chin Pharm J(中国药学杂志), 2017, 52(9):743-749.
[10] RU J L. Construction and utilization of traditional Chinese medicine systems pharmacology database and analysis platform[D]. Xi′an:Shaanxi University of Chinese Medicine, 2015.
[11] XIA H L, JIANG Y Y, SHI R B.Quality control of effective fraction in baihe zhimu decoction[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2013, 19(4):126-130.
[12] LUO J, SHI S H, ZHANG L Q, et al. UPLC Determination of six active components including mangiferin in Anemarrhenae rhizoma[J]. Chin Pharm J(中国药学杂志), 2012, 47(22):1856-1859.
[13] WANG J J, LOU Z Y, WU Y T. Determination of mangiferin and neomangiferin in Rhizoma Anemarrhenal using RP-HPLC[J]. Chin Pharm J(中国药学杂志), 2004, 39(9):657-658.
[14] ZHANG Y, CHEN C, CHEN Y X, et al. Study of antidepressant on Anemarrhena asphodeloides Bge[J]. Glob Chin Med(环球中医药), 2016, 9(3):377-380.
[15] LUO H, CHEN J, SHI L M, et al. DRAR-CPI:a server for identifying drug repositioning potential and adverse drug reactions via the chemical-protein interactome[J]. Nucleic Acids Res, 2011, 39:492-498.
[16] CAO W Y, FENG B, WANG X J. Studies progress on metabolism of natural saponins by intestinal flora /liver drug enzyme[J]. J Chin Pharm(中国药房),2016,27(28):3999-4002.
[17] FU Z W. Timosaponin in vivo metabolism and metabolic product research[D]. Shanghai:Shanghai University of Traditional Chinese Medicine, 2015.
[18] ZHENG S Q. Theoretical and experimental studies on the anti-depression effect of Baihe-Zhimu decoction[D]. Shanghai:The Second Military Medical University, 2007.
[19] CAI F, WANG W H, GAO S H, et al. Advances in the pharmacological studies on timosaponins and their sapogenins[J]. J Pharm Pract(药学实践杂志), 2011, 29(5):331-334.
[20] LU M Z, ZHANG Z Q, YI J, et al. Study on the effect and mechanisms of timosaponin BⅡ on antidepressant[J]. J Pharm Pract(药学实践杂志), 2010, 28(4):283-287.
[21] ZHOU H W, ZHANG H S, ZHU H F, et al. Effects of emodin on depress-derived behavioral changes and brain monoamine neurotransmitters in rats[J]. Acta Med Univ Sci Technol Huazhong(华中科技大学学报·医学版), 2017, 46(2):150-154.
[22] FU C Y, LIU Y H, ZENG L, et al. Research progress of antidepressant efficacy of lily[J]. Guid J Tradit Chin Med Pharm(中医药导报), 2015, 21(20):83-86.
[23] SUN L, SUN H W, SONG Y P, et al. Research progress of the mtor signaling pathway underlying the antidepressant actions of ketamine[J]. Chin Pharm J(中国药学杂志), 2015, 50(8):653-657.
[24] HU Q, WANG L, JIANG W, et al. Effect of ketamine on c-fos gene expression in neuronal PC12 cells line induced by glutamate[J]. J Shanghai Jiaotong Univ Med Sci(上海交通大学学报:医学版), 2006, 26(2):159-162.
[25] GAO Y, GAO L, GAO X F, et al. An exploration in the action targets for antidepressant bioactive components of Xiaoyaosan based on network pharmacology[J]. Acta Pharm Sin(药学学报), 2015, 50(12):1589-1595.
[26] ZOU H Y, YANG Y L, TU H J, et al. Effects of TCM compound of resolving stagnation for tranquilization on neurotransmitters and brain-derived neurotrophic factors in depression rat model by chronic unpredictable stress[J]. J Beijing Univ Tradit Chin Med (北京中医药大学学报), 2015, 38(9):611-618.
[27] REN L X, LUO Y F, GAO W, et al. Protective effect of total timosaponin on chronic mildly stressed mice and its mechanism[J]. Tradit Chin Drug Res Pharmacol(中药新药与临床药理), 2011, 22(4):414-417.
[28] GUO Z K, XUE H, WANG W W, et al. Effect of nourishing yin and clearing heat therapy on monoamine neural transmitter, brain-deried neurotrophic factor, tyrosine kinase B in depression rat model′s brain[J].Chin Arch Tradit Chin Med(中华中医药学刊), 2014, 32(8):1943-1948.
[29] ZHOU J, ZHANG L, XI H L, et al. Meta analysis of Xiaoyaosan treatment of type 2 diabetes combined with depression[J]. Acta Chin Med(中医学报), 2017, 32(10):1878-1882.
[30] DELA FUENTE J R, ROSENBAUM A H. Prolactin in psychiatry[J]. Am J Psychiatry, 1981, 138(9):1154-1160.
[31] GUO Q P, GAO Y, LI W M. Lily effective parts within the depression model rats on single amine neurotransmitter[J]. Chin Tradit Pat Med(中成药), 2009, 31(11):1669-1672.
[32] GUO Q P, GAO Y, LI W M. Influence of the lilium saponins on HPA axis of the depression model rats[J]. Chin Pharmacol Bull(中国药理学通报), 2010, 26(5):699-700.
[33] POLTER A, YANG S, ZMJIEWSKA A A, et al. Forkhead box, class O transcription factors in brain:regulation and behaviormanifestation[J]. Biol Psychiatry, 2009, 65(2):150-159.
[34] LU J, YANG X Y, HUA Q, et al. cAMP Response element binding protein:antidepressants intersection signal transduction pathways[J]. Prog Phys Sci (生理科学进展), 2008, 39(4):371-374.
[35] MENG P, DU Q, ZHANG X L, et al.Effects of Baishile Capsules on hippocampal PI3K signaling pathway in chronic mild unpredictable stress rats[J]. Chin J Inf Tradit Chin Med(中国中医药信息杂志), 2017, 24(8):41-45.
[36] WANG L J, JIN Y, SUI H J, et al.Protection of sarsasapogenin against amyloid beta-protein induced neurotoxicity in primary cultured hippocam palneurons of neonatal rats[J]. Chin J Pharmacol Toxicol(中国药理学与毒理学杂志), 2013, 27(4):629-634.
[37] XIAO F Q, LI H X, SUI H J, et al.Protective effect of sarsasapogenin against hyperglycemia induced neurotoxicity in cultured hippocampal neurons[J]. Chin Pharmacol Bull(中国药理学通报), 2013, 29(1):107-112.
[38] WANG Q, SUI H J, QU W H, et al.Protective effects of sarsasapogenin against glutamate-induced neurotoxicity in the cultured cortical neurons in rats[J]. Chin Pharmacol Bull(中国药理学通报), 2013, 29(2):281-285.
[39] LI N.Change mechanism of hippocampal angiogenesis in chronic stress elderly depressive rats and intervention effect of venlafaxine[D]. Kunming:Kunming Medical University, 2013.

基金

江西省教育厅项目(GJJ12596)
PDF(3198 KB)

79

Accesses

0

Citation

Detail

段落导航
相关文章

/