栀子豉汤提取物对谷氨酸诱导PC 12细胞损伤的保护作用

姚媛, 果卉, 马鑫, 李腾, 周婷婷

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (12) : 982-987.

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PDF(1788 KB)
中国药学杂志 ›› 2018, Vol. 53 ›› Issue (12) : 982-987. DOI: 10.11669/cpj.2018.12.009
·论著·

栀子豉汤提取物对谷氨酸诱导PC 12细胞损伤的保护作用

  • 姚媛1,2, 果卉1, 马鑫1, 李腾1,3, 周婷婷1*
作者信息 +

The Protective Effect of Zhizichi Decoction on the Apoptosis of PC 12 Cells Induced by Glutamic Acid

  • YAO Yuan1,2, GUO Hui1, MA Xin1, LI Teng1,3, ZHOU Ting-ting1*
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文章历史 +

摘要

目的 研究栀子豉汤提取物对谷氨酸诱导 PC 12细胞损伤的保护作用。方法 通过谷氨酸诱导PC 12细胞建立细胞损伤模型,采用四甲基偶氮唑蓝(MTT)比色法检测细胞存活率;化学检测法测定乳酸脱氢酶(LDH)释放量;Annexin V-FITC/propidium iodide(PI)双染法流式细胞术(flow cytometry,FCM)检测细胞凋亡率;流式细胞仪检测PC 12细胞内活性氧(ROS)水平,酶标仪检测超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的含量,初步探讨栀子豉汤提取物的抗氧化机制。结果 栀子豉汤可显著降低由谷氨酸诱导的细胞损伤,提高细胞存活率,减少LDH释放量,增加 SOD、GSH活性,抑制ROS的生成。结论 栀子豉汤提取物具有减轻由谷氨酸诱导的PC 12细胞损伤的作用,其机制可能与抗氧化应激损伤有关。

Abstract

OBJECTIVE To explore the protective effect of Zhizichi Decoction extract against glutamic acid-induced neurotoxicity in PC 12 cells. METHODS PC 12 cells were treated with glutamic acid to establish the cell model. Cell viability and membrane permeability were examined by MTT assay and LDH kit. The apoptosis rates were measured by flow cytometry using fluorescein labeling FITC-Annexin V/PI. The production of ROS was determined by flow cytometry. The levels of GSH and SOD in the cells were determined by kit respectively. RESULTS Zhizichi Decoction extract significantly decreased the contents of LDH, enhanced the activities of SOD and GSH induced by glutamate. In addition, Zhizichi Decoction extract treatment decreased the intracellular ROS generation. CONCLUSION Zhizichi Decoction extract can significantly ameliorate glutamic acid induced PC 12 cells injury. The mechanism may be partly through anti-oxidative stress.

关键词

栀子豉汤提取物 / PC 12细胞 / 谷氨酸 / 神经保护作用

Key words

Zhizichi Decoction / PC 12 cells / glutamic acid / neuroprotective effect

引用本文

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姚媛, 果卉, 马鑫, 李腾, 周婷婷. 栀子豉汤提取物对谷氨酸诱导PC 12细胞损伤的保护作用[J]. 中国药学杂志, 2018, 53(12): 982-987 https://doi.org/10.11669/cpj.2018.12.009
YAO Yuan, GUO Hui, MA Xin, LI Teng, ZHOU Ting-ting. The Protective Effect of Zhizichi Decoction on the Apoptosis of PC 12 Cells Induced by Glutamic Acid[J]. Chinese Pharmaceutical Journal, 2018, 53(12): 982-987 https://doi.org/10.11669/cpj.2018.12.009
中图分类号: R965   

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基金

国家自然科学基金项目资助(81573584,81773862)
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