目的 探究滇黄精对高脂饮食诱导的非酒精性脂肪肝大鼠的保护作用及其机制。方法 42只SD大鼠随机分为正常对照(生理盐水)组、模型(生理盐水)组、白藜芦醇组(阳性对照,40 mg·kg-1)和滇黄精水提物低、中、高剂量组(1、4、8 g·kg-1),连续灌胃给药14周,每天1次。除正常对照组外其余各组大鼠喂高脂饲料14周诱发非酒精性脂肪肝。于0、6、12、14周眼角取血,检测血清甘油三酯(TG)和总胆固醇(TC)水平;并于14周处死大鼠,测定脏器(肝、脾、肾)指数,检测肝脏中TC、TG、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)水平,检测大鼠肝脏线粒体中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、ATP合酶及呼吸链复合物Ⅰ、Ⅱ水平。结果 高脂饲料喂养大鼠被成功诱发非酒精性脂肪肝。滇黄精水提物各剂量组可显著抑制高脂饮食诱导的大鼠肝脏指数及血清TC升高,减轻肝细胞肿胀、变性、坏死及炎性损伤,抑制肝脏中TC、TG、LDL-C升高及HDL-C降低,抑制肝线粒体中MDA升高及SOD、GSH-PX、ATP合酶与呼吸链复合物Ⅰ、Ⅱ活性降低。结论 滇黄精对高脂饮食诱导的非酒精性脂肪肝大鼠具有保护作用,其机制可能与清除线粒体氧化应激产物MDA,增加线粒体内抗氧化酶SOD 与GSH-PX活性及改善能量代谢障碍有关。
Abstract
OBJECTIVE To investgate the protective effects and mechanisms of Polygonatum kingianum on nonalcoholic fatty liver induced by high-fat diet in rats. METHODS A total of 42 rats were randomly divided into normal control group (normal saline), model group (normal saline), resveratrol group (positive control, 40 mg·kg-1) and low-, middle-and high-dose P. kingianum group (1, 4, 8 g·kg-1). They were intragastrically given corresponding compounds (or normal saline) once a day, lasting for 14 weeks. Nonalcoholic fatty liver was induced by feed with high-fat diet for 14 weeks in those groups except for normal control group. Blood was taken from the corneas at 0, 6, 12 and 14 week, and then the levels of triglyceride (TG) and total cholesterol (TC) in serum were determined. Afterword, the rats were sacrificed at 14 week followed by the measurement of organs indices of liver、spleen and kidney, as well as the detection of levels of TC, TG, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in liver tissues. Furthermore, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), ATP synthase and respiratory chain complex Ⅰ and Ⅱ in hepatic mitochondria were determined. RESULTS Nonalcoholic fatty liver was successfully induced by high-fat diet in rats. The different doses of water extract of P. kingianum could significantly inhibit the increasing of liver index and serum TC in high-fat diet-induced rats, alleviate swelling, degeneration, necrosis and inflammatory injury of hepatic cells, inhibit the increasing of TC, TG and LDL-C and decrease of HDL-C in liver tissues, as well as inhibit the exaltation of MDA level and reduction of SOD, GSH-PX, ATP synthase and respiratory chain complex Ⅰ and Ⅱ activity in hepatic mitochondria. CONCLUSION P. kingianum shows protective effects on high-fat diet-induced nonalcoholic fatty liver in rats. The action mechanism may be related to the elimination of oxidative stress product (MDA) and improvement of antioxidant enzyme activity (SOD and GSH-PX) in hepatic mitochondria, as well as the improvement of energy metabolism obstacle.
关键词
滇黄精 /
非酒精性脂肪肝 /
保护作用 /
氧化应激 /
线粒体 /
能量代谢
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Key words
Polygonatum kingianum /
nonalcoholic fatty liver /
protective effect /
oxidative stress /
mitochondria /
energy metabolism
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中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金项目资助(81660596,81460623,81760733);云南省应用基础研究计划项目资助(2017FF117-(013),2016FD050);云南省刘昌孝院士工作站资助;云南省中青年学术和技术带头人后备人才项目资助(2015HB053);云南省高校南药研究协同创新中心项目资助(30270100500)
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