1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China 2. Hebei Traditional Chinese Medicine Formula Granule Engineering & Technology Research Center, Shijiazhuang 050091, China 3. Traditional Chinese Medicine Formula Granule Research Center of Hebei Province University, Shijiazhuang 050091, China 4. Shineway Pharmaceutical Group Ltd., Shijiazhuang 051430, China
Abstract��OBJECTIVE To establish an HPLC method to simultaneously determine the contents of geniposidic acid, caffeic acid, acteoside and isoacteoside in Plantaginis Semen formula granules, in order to provide basis for studying its quality standards. METHODS An HPLC method was developed. Kinetex C18 column (2.1 mm��100 mm,2.6 ��m)was used and eluted with mobile phase of 0.5% acetic acid-acetonitrile at the flow rate of 0.3 mL��min-1. The wavelength was set at 239 nm for geniposidic acid, 325 nm for caffeic acid, and 330 nm for acteoside and isoacteoside. The column temperature was maintained at 30 ��. RESULTS The good linear relationships between the concentration and peak area were in the range of 0.292 1-2.92 1 ��g for geniposidic acid, 0.003 4-0.033 6 ��g for caffeic acid, 0.047 6-0.476 ��g for acteoside and 0.102 7-1.027 ��g for isoacteoside (r��0.999 8), respectively. The average recoveries were 99.32%, 98.62%, 98.23% and 98.51% with RSDs of 1.47%, 1.36%, 1.62% and 1.53%, respectively. CONCLUSION The method is simple, feasible and reproducible and can be used for the quality control of Plantaginis Semen formula granules.
SUN Y Y, SHI P. Countermeasures for promoting internationalization of Chinese materia medica by virtue of formula granule [J]. Chin Tradit Herb Drugs (�в�ҩ), 2013, 44(8): 929-934.
TIAN W, ZHEN Y Q, CAO W L, et al. Simultaneous determination of saikosaponin a, b1, b2 and c in Radix Bupleuri Formula Granules by HPLC-MS/MS[J]. Chin Pharm J (�й�ҩѧ��־), 2016, 51(23): 2068-2071.
TIAN Y R, ZHEN Y Q, WANG X G, et al. Assay and fingerprint chromatogram of Scutellaria barbata Formula Granules by HPLC[J]. Chin Pharm J (�й�ҩѧ��־), 2016, 51(20): 1795-1799.
ZHENG X J,YANG L, WANG Z T. Advances in studies on chemical compositions and pharmacological activities of Plantaginis Semen[J]. J Chin Med Mater(��ҩ��), 2013, 36(7): 1190-1196.
XU B B, HUANG B T, ZENG J X, et al. Comparison of contents of verbascoside and isoverbascoside in Plantaginis Semen and Plantaginis Herba[J]. Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־), 2016, 22(14): 58-61.
LU D Y, HUANG W R, MA Z G. Meeting notes of academic annual meeting for Science of processing Chinese materia medica together with Chinese herbal medicine innovation and development forum and collaborative innovation alliance conference on 2014 [C]. Nanjing:2014:176.
WAN Y, XIE M Y, HE Y L. Determination of verbascoside and isoverbascoside in Semen Plantaginis by RP-HPLC[J]. Nat Prod Res Dev(��Ȼ�����о��뿪��), 2008, 20(3): 474-476.
XU L, TIAN J X, SONG R, et al. LC-MS/MS Determination and comparison of saikosaponin a, b2, c, d in crude and processed Radix Bupleuri by vinegar[J]. Chin Pharm Univ J(�й�ҩ�ƴ�ѧѧ��), 2012, 43(4): 334-340.
YANG S D, HU J H, LI J C, et al. Simultaneous determination of echinacoside, acteoside and isoacteoside in total Cistanchis Glycosides Capsules by HPLC [J]. Mod Tradit Chin Med Mater Med World Sci Tech(�����ѧ����-��ҽҩ�ִ���), 2015, 17(3): 609-613.