目的 建立反相高效液相色谱法同时测定全缘叶蓝刺头中两种黄酮醇化合物——柯伊利素和矢车菊黄素的含量。方法 采用Kromasil C18色谱柱( 4.6 mm×250 mm,5 μm),流动相为乙腈-水-冰乙酸( 35∶65∶2),流速1.0 mL·min-1,检测波长为350 nm,柱温为30 ℃。结果 柯伊利素和矢车菊黄素在进样质量浓度分别为0.4~2.4、0.6~2. 6 mg·L-1内呈良好线性关系,相关系数分别为0.999 8和0.999 9,加样回收率(n=6)分别为91.6%和92.7%,RSD分别为1.37%和1.08%。结论 该方法经方法学验证可用于全缘叶蓝刺头样品中柯伊利素和矢车菊黄素的含量测定。
Abstract
OBJECTIVE To establish an RP-HPLC analytical method for simultaneous determination of crysoeriol and centaureidin in the aeries parts of Echinops integrifolius. METHODS The separation was achieved on a Kromasil C18 column ( 4. 6 mm×250 mm,5 μm)at 30 ℃ using acetonitrile-water-acetic acid solution (35∶65∶2) as mobile phase at a flow rate of 1.0 mL·min-1 and 350 nm as the detection wavelength. RESULTS The linear ranges of crysoeriol and centaureidin were 0.4-2.4 mg·L-1(r=0.999 8), 0.6-2. 6 mg·L-1 (r=0.999 9), respectively. The average recoveries (n=6) were 91.6% and 92.7%, and RSDs were 1.37% and 1.08% respectively. CONCLUSION The methodology validation shows that this method is accurate,simple and reliable,which is applicable for the simultaneous determination of two flavonols crysoeriol and centaureidin in the aeries parts of Echinops integrifolius.
关键词
全缘叶蓝刺头 /
柯伊利素 /
矢车菊黄素 /
高效液相色谱法 /
含量测定
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Key words
Echinops integrifolius /
crysoeriol /
centaureidin /
RP-HPLC /
content determination
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中图分类号：
R284
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参考文献
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脚注
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基金
国家“重大新药创制”项目资助(2011ZX09307-002-01);国家自然科学基金项目资助(31000160);辽宁省自然科学基金项目资助(2013020127);新疆科技厅-新疆少数民族科技人才特殊培养计划科研项目资助(201223111);新疆科技厅-国家高层次人才特殊支持计划后备人选培养项目资助(科技创新领军人才后备人选项目);新疆科技厅-自治区青年科技创新人才培养工程项目-杰出青年科技人才培养项目资助(2013711020)
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