1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China 2. Xinjiang Institute of Materia Medica, Xinjiang Institute of Kazak Medicine, Urumqi 830002, China 3. College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
Abstract��OBJECTIVE To establish an RP-HPLC analytical method for simultaneous determination of crysoeriol and centaureidin in the aeries parts of Echinops integrifolius. METHODS The separation was achieved on a Kromasil C18 column ( 4. 6 mm��250 mm,5 ��m)at 30 �� using acetonitrile-water-acetic acid solution (35��65��2) as mobile phase at a flow rate of 1.0 mL��min-1 and 350 nm as the detection wavelength. RESULTS The linear ranges of crysoeriol and centaureidin were 0.4-2.4 mg��L-1(r=0.999 8), 0.6-2. 6 mg��L-1 (r=0.999 9), respectively. The average recoveries (n=6) were 91.6% and 92.7%, and RSDs were 1.37% and 1.08% respectively. CONCLUSION The methodology validation shows that this method is accurate,simple and reliable,which is applicable for the simultaneous determination of two flavonols crysoeriol and centaureidin in the aeries parts of Echinops integrifolius.
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MAKABEL Bolat,MENG Fan-tong,KONG Liang, ZHU Shen-tong, LI Wei,PEI Di,AILANKAN Munira,GU Zheng-yi,TAN Cheng-yu,ZOU Zhong-mei. Simultaneous Determination of Contents of Two Flavonols in the Aerial Parts of Echinops integrifolius by RP-HPLC. Chinese Pharmaceutical Journal, 2018, 53(2): 136-139.
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