Rapid Quality Evaluation of Huoxiang Zhengqi Tincture (��): Simultaneous Determination of Seven Components in Huoxiang Zhengqi Tincture by UPLC with Wavelength Switching Detection
NIE Li-xing1,HE Yu-qing2,YU Jian-dong1, DAI Zhong1*,MA Shuang-cheng1*
1. National Institutes for Food and Drug Control, Beijing 100050, China; 2. Jining Mdedical University, Rizhao 276800, China
Abstract��OBJECTIVE To investigate a rapid approach for quality evaluation of Huoxiang Zhengqi Tincture. METHODS Hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin in Huoxiang Zhengqi Tincture were determined by ultra performance liquid chromatography (UPLC) method combined with wavelength switching detection technology. The sample was injected directly without preprocessing. The separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm��100 mm, 1.8 ��m) and the column temperature was maitained at 40 ��. The mobile phase was composed of acetonitrile and 0.1% phosphoric acid with gradient elution at a flaw rate of 0.3 mL��min-1. Hesperidin was detected at 284 nm; glycyrrhizic acid was detected at 250 nm; imperatorin, honokiol, isoimperatorin and magnolol were detected at 300 nm; atractylodin was detected at 340 nm. RESULTS The calibration curves of the seven components showed good linearity within their test ranges. The average recoveries for hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin were 99.3%, 99.5%, 101.5%, 99.3%, 100.6%, 99.0% and 99.6%, respectively. And there were great variations among the contents of glycyrrhizic acid, imperatorin, isoimperatorin and atractylodin in 28 batches of samples from 13 manufactures. CONCLUSION The proposed method is accurate, simple and rapid, thus providing basis for comprehensive quality control of Huoxiang Zhengqi Tincture.
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2017, Vol. 52 
