Purification and Identification of Chemical Constituents of Pedicularis longiflora Rudolph.var. Tubiformis (Klotz). Tsoong
DENG Yu1, WANG Qiu-ru2, WANG Jian-hua1,3, TANG Hong-mei1, LI Cheng-hong1*
1. Chongqing Academy of Animal Sciences, Chongqing 402460, China; 2. School of Medicine, Shandong University, Jinan 250012, China; 3. School of Biological Engineering, Chongqing University, Chongqing 400044, China
Abstract��OBJECTIVE To investigate the chemical constituents of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong systematically. METHODS Silica-gel column, reversed phase C18 column, macroporous resin column and Sephadex LH-20 column were employed to purify the chemical constituents. The ability to remove DPPH free radicals was determined to evaluate the antioxidant activities of the crude extract, eluted fractions and compounds. RESULTS Ten compounds were obtained and identified as (E)-3-(��-D-glucopyranosyloxy)-5-hydroxy-4��-methoxystilbene (1), ��-sitosterol (2), iridotrial glucoside (3), luteolin(4), 5,7,4��-trihydroxy-3��,5��-dimethoxyflavone (5), echinacoside (6), jionoside A1 (7), kankanoside H1 (8), kankanoside l (9) and pedieularioside I (10) based on the NMR spectra together with physical and chemical properties. In addition, the methanol-eluted fractions and compound 6 showed moderate antioxidant activity. CONCLUSION Compounds 1, 3 and 6-10 are isolated form Pedicularis longiflora Rudolph.var. tubiformis (Klotz).Tsoong for the first time.
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DENG Yu, WANG Qiu-ru, WANG Jian-hua, TANG Hong-mei, LI Cheng-hong. Purification and Identification of Chemical Constituents of Pedicularis longiflora Rudolph.var. Tubiformis (Klotz). Tsoong. Chinese Pharmaceutical Journal, 2017, 52(24): 2146-2150.
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2017, Vol. 52 
