Abstract��OBJECTIVE To develop an ultra-high performance liquid chromatography(UHPLC) method for simultaneous determination of berberine hydrochloride, phellodendrine hydrochloride, palmatine hydrochloride, magnoflorine, and rhizoma atractylodis in Ermiao pills. METHODS The UHPLC analyses were performed on a Waters BEH C18 column (2.1 mm��100 mm,1.7 ��m)eluted with acetonitrile and 0.1% phosphoric acid(each 100 mL containing 0.1 g sodium dodecyl sulfate). The flow rate was 0.3 mL��min-1, the detection wavelength was set at 280 and 340 nm, and the column temperature was set at 30 ��. RESULTS The calibration curves of the five compositions had good linear relationship in the ranges of the tested concentrations. The precision, stability and repeatability complied with the requirements of methodology. The recoveries were between 96.8%-99.3% respectively. The RSDs were below 2.8%. CONCLUSION The developed method is simple, sensitive, rapid and accurate. This work provides helpful information for the comprehensive quality evaluation of Ermiao pills.
�����,����,����Ƽ,������. ����ЧҺ��ɫ��˫������ͬʱ�ⶨ��������5�ֳɷֵĺ���[J]. �й�ҩѧ��־, 2017, 52(23): 2122-2125.
ZHOU Ya-nan, LEI Rong, KONG Ya-ping, LIU Yong-li. Simultaneous Determination of Five Components in Ermiao Pills by UHPLC with Dual-Wavelength. Chinese Pharmaceutical Journal, 2017, 52(23): 2122-2125.
ZHANG H H, SUN Z S. Methods of double wavelengths fusion and HPLC to determine berberine hydrochloride and atractylodin in Ermiao pill[J] . J Liaoning Univ TCM(������ҽҩ��ѧѧ��),2015,17(6):53-55.
[2]
PAN C, ZHANG L, WANG Y. Simultaneous determination of phellodendrine,magnoflorine,jatrorrhizine,palmatine hydrochloride and berberine hydrochloride in Ermiao pill by HPLC[J] . Chin J Biochem Pharm(�й�����ҩ����־) ,2012,33(4):361-364.
[3]
ZHANG L, PAN C. Simultaneous determination of berberine hydrochloride and phellodendrine in Ermiao Wan by HPLC[J] . Chin J New Drugs(�й���ҩ��־),2011,20(22):2262-2264.
[4]
FAN X Y,WAN L Y,YUAN L P,et al. Determination of jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride in Ermiao pill by HPLC[J] . Chin Pharm J(�й�ҩѧ��־),2006,41(12):942-944.
[5]
CHEN J H,WANG Z G,JIANG X L,et al. The determination of rhizoma atractylodis in Ermiao pill [J] . Her Med(ҽҩ����),2014,33(3):380-381.
[6]
ZHANG Z S,ZHOU L,CAO A M,et al. Study on quality standard of Ermiao pills [J] . Liaoning J Tradit Chin Med(������ҽ��־),2009,36(4):606-607.
[7]
ZHANG Y,ZHANG Z Q,WANG H B,et al. Quality study of multi-chemical compositions in Ermiao pill [J] . Chin Tradit Pat Med(�г�ҩ),2009,31(4):562-566.
[8]
LI D D,JIANG P,YANG S L,et al. The research progress of chemical component, pharmacological action and clinical application in golden cyperss[J] . Heilongjiang Med J(������ҽҩ),2014,27(3):601-604.
[9]
ZHOU S,LIU Y G,ZHANG G X,et al. The research progress of chemical component and quality control in golden cyperss[J] . J China Pharm(�й�ҩ��),2012,23(39):3740-3741.
[10]
DENG L M,LI Y,WU Z T,et al. Advances in studies on chemical compositions of atractylodes lancea and their biological activities [J] . China J Chin Mater Med(�й���ҩ��־),2016,41(21):3904-3913.
[11]
LI W J,GUO Y L, SHANG C L,et al. Analysis of chemical constituents of tractylodes Chinensis Rhizome by GC-MS [J] . Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־),2016,22(6):66-70.
[12]
ZHU C C,LIN Z Z,MO J X,et al. Determination of berberine and phellodendrine in Cortex Phellodendri by HPLC [J] . Tradit Chin Drug Res Clin Pharmacol(��ҩ��ҩ���ٴ�ҩ��),2004,15(4):262-264.
[13]
MA R R,TANG Y H,MA C H,et al. Determination of berberine and palmatine in Golden Cyperss of different producing areas by HPLC-UV [J] . Heilongjiang Med J(������ҽҩ),2007,20(6):560-562.
2017, Vol. 52 
