Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics
ZHANG Zhi-min, HU Yu-zhen, XIONG Su-hui, TANG Jie, LI Qiang, LEI Si-min, XIA Bo-hou*, WU Ping*
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Pharmacy of Hunan University of Chinese Medicine, Changsha 410208, China
Abstract��OBJECTIVE To evaluate the effect of supernatants of alcohol extracting-water precipitation of Polygonatum odoratum(POS) on hyperthyroidism-yin deficiency rats. METHODS SD rats were randomly divided into control, model and POS groups(400 mg��kg-1). The hyperthyroidism-yin deficiency rats were treated by intraperitoneal injection of T3(500 ��g��kg-1) for a week. The content of T3��T4 in serum and cAMP��cGMP in plasma was determined. A GC-MS based metabolic profiling method was employed to analyze the distinctive metabolic patterns of serum in control, model and POS. Multivariate statistical analysis was applied for multivariate analysis to find potential biomarkers of hyperthyroidism-yin deficiency by SIMCA-P 12.0 software. RESULTS Compared with the model group, POS group can significantly reduce the content of T3, T4, cAMP, the ratio of cAMP/cGMP and increase the content of cGMP, indicating it has certain Yin-nourishing action. The PCA and PLS-DA score plots showed metabolic profiling of control, model and POS had significant differences, but which could be regulated nearly to normal state after POS interference. Four potential biomarkers were identified by PLS-DA and variable importance for project values(VIP) based on the metabolomics. They were 3-hydroxybutyric acid, galactose, palmitic acid and cholesterol. CONCLUSION POS may play a role in treating hyperthyroidism-yin deficiency rats, by adjusting energy metabolism, glucose metabolism and lipid metabolism. The changes of metabolic pathways revealed by this study may provide some basis for the diagnosis and treatment of hyperthyroidism Yin-deficiency.
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ZHANG Zhi-min, HU Yu-zhen, XIONG Su-hui, TANG Jie, LI Qiang, LEI Si-min, XIA Bo-hou, WU Ping. Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics. Chinese Pharmaceutical Journal, 2017, 52(23): 2092-2097.
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2017, Vol. 52 
