Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics
ZHANG Zhi-min, HU Yu-zhen, XIONG Su-hui, TANG Jie, LI Qiang, LEI Si-min, XIA Bo-hou*, WU Ping*
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Pharmacy of Hunan University of Chinese Medicine, Changsha 410208, China
Abstract��OBJECTIVE To evaluate the effect of supernatants of alcohol extracting-water precipitation of Polygonatum odoratum(POS) on hyperthyroidism-yin deficiency rats. METHODS SD rats were randomly divided into control, model and POS groups(400 mg��kg-1). The hyperthyroidism-yin deficiency rats were treated by intraperitoneal injection of T3(500 ��g��kg-1) for a week. The content of T3��T4 in serum and cAMP��cGMP in plasma was determined. A GC-MS based metabolic profiling method was employed to analyze the distinctive metabolic patterns of serum in control, model and POS. Multivariate statistical analysis was applied for multivariate analysis to find potential biomarkers of hyperthyroidism-yin deficiency by SIMCA-P 12.0 software. RESULTS Compared with the model group, POS group can significantly reduce the content of T3, T4, cAMP, the ratio of cAMP/cGMP and increase the content of cGMP, indicating it has certain Yin-nourishing action. The PCA and PLS-DA score plots showed metabolic profiling of control, model and POS had significant differences, but which could be regulated nearly to normal state after POS interference. Four potential biomarkers were identified by PLS-DA and variable importance for project values(VIP) based on the metabolomics. They were 3-hydroxybutyric acid, galactose, palmitic acid and cholesterol. CONCLUSION POS may play a role in treating hyperthyroidism-yin deficiency rats, by adjusting energy metabolism, glucose metabolism and lipid metabolism. The changes of metabolic pathways revealed by this study may provide some basis for the diagnosis and treatment of hyperthyroidism Yin-deficiency.
������,������,���ջ�,�ƽ�,��ǿ,��˼��,�Ĳ���,��Ƽ. ����ˮ�ᴼ������Һ�Լ������ʹ����Ԥ��GC-MS��л��ѧ�о�[J]. �й�ҩѧ��־, 2017, 52(23): 2092-2097.
ZHANG Zhi-min, HU Yu-zhen, XIONG Su-hui, TANG Jie, LI Qiang, LEI Si-min, XIA Bo-hou, WU Ping. Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics. Chinese Pharmaceutical Journal, 2017, 52(23): 2092-2097.
ZHANG W. Pharmacodynamics Study of Yinning Tablets on the Rats with Yin Deficiency Hyperthyroidism[D] . Guangzhou: Southern Medical University, 2007.
ZHONG G S. Chinese Materia Medica(��ҩѧ)[M] . 3rd ed. Beijing: China��s Traditional Chinese Medicine Press,2012:411.
LIU M. Kinetic Model of Extraction and Structural Characterization of Water-Soluble Polysaccharide from Polygonatum odoratum[D] . Guangzhou: Guangdong Pharmaceutical University, 2015.
XU L L, ZHAN X R, ZENG S W,et al. The research progress of polygonatum polysaccharides[J] . J Chin Med Mater(��ҩ��),2011,34(1):154-157.
GUO H J. Studies on the Steroid Saponins and Activities of Polygonatum odoratum(Mill.) Druce[D] . Jinan: University of Jinan, 2013.
LAN G S. Studies on the physicochemical properties and bioactivities of polysaccharides from polygonatum odoratum [D] . Tianjin: Tianjin University, 2012.
ZHOU L. Yin jade bamboo is good for lipid-lowering [J] . Orient Med Diet(����ҩ��),2008,(3):43.
ZHU P H, CHEN A P. The component analysis of volatile oil from Hunan Polygonatum odoratum(Mill.) Druce by GC-MS [J] . Chin Tradit Herb Drugs(�в�ҩ),2010,41(8):1264-1265.
GE Z P. The introduction of Polygonatum odoratum(Mill.) Druce about nourishing Yin and generating body fluid[J] . Mod Health(�ִ�����),2013,11:29-31.
REN H Y, XUE C M, ZHANG Y, et al. Experimental study of the effects of Huangjing Polysaccharide for Yin-deficient model mouse caused by drugs with warm nature [J] . Shandong J Tradit Chin Med(ɽ����ҽ��־),2005,34(1):36-37.
RONG L X, LU S, LIU Y M. Immunomodulatory effects of Radix Glehniae polysaccharide on Yin Deficiency hyperthyroidism mice[J] . Chin J Basic Med Tradit Chin Med(�й���ҽ����ҽѧ��־),2013,19(6):640-641.
MIAO M S. Studies on nourishing Yin capacity of(Huai) Radix Rehmaniae preparate polysaecharide [J] . J Henan Coll Tradit Chin Med(������ҽҩѧ��),1997,34(4):24-25.
WANG P, WANG X J. A survey of studies on kidney-Yin Deficiency animal model[J] . Inf Tradit Chin Med(��ҽҩ��Ϣ),2013,34(4):123-125.
ESCOBAR-MORREALE H F, SAMINO S, INSENSER M, et al. Metabolic heterogeneity in polycystic ovary syndrome is determined by obesity: plasma metabolomic approach using GC-MS[J] . Clin Chem, 2012,58(6): 999-1009.
KONG X Y, ZOU J L, HUANG W Y. Experimental study on immuno-regulating effect of Astragalus membranaceus and Angelica sinensis [J] . J Shandong Univ Tradit Chin Med(ɽ����ҽҩ��ѧѧ��),1997,21(4):266.
HUANG J R, LI X H, ZHANG J J, et al. Effects of Liuwei Dihuang Wan on content of cAMP��cGMP and activity of Na+, K+-ATPenzyme in hyperthyroidism and Shen Yin deficiency model animals by tetraiodothyronine and reserpine[J] . Pharm Clin Chin Mater Med(��ҩҩ�����ٴ�),2011,(6):1-3.
KUANG A K, DING T, DENG J L, et al. The comparison of blood plasma cyclic nucleotide levels in Yang Xu(hypothyroidism) patients and Yin Xu(hyperthyroidism) patients[J] . J Tradit Chin Med(��ҽ��־),1979,(7):21-24.
XIA Z Q, ZHU M, HU Y E L,et al. A preliminary study of the theory of ��Deficiency Syndrome�� in TCM(changes of blood plasma cyclic nucleotide levels in Yang Xu and Yin Xu patients) [J] . J Tradit Chin Med(��ҽ��־),1979,(11):2-10.
WANG B, SHEN L, CONG W J, et al. Serum metabonomic study on Liuwei Dihuang Pills in the treatment of hyperthyroidism[J] . Chin Pharmacol Bull(�й�ҩ��ѧͨ��),2013,29(5):632-637.