Hypoglycemic Effect of Total Flavonoids from Ampelopsis grossedentata
QI Jiao-mei1, JIANG Yan-qun1, ZHANG Jie1, YANG Yi-han1, FU Ming1,2,3*
1. College of Biological and Food Engineering,Huaihua University, Huaihua 418008,China; 2. Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua 418008, China; 3.Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education,Huaihua 418008, China
Abstract��OBJECTIVE To investigate the effects of total flavones of Ampelopsis(TFAG) on blood glucose and its antioxidant capacity. METHODS The TFAG were extracted by ultrasonic assisted extraction and purified by D-101 macroporous resin. The effect of TFAG on the activities of ��-glucosidase and ��-amylase were determined in vitro. Diabetes mellitus(DM)mouse models were established by induction of streptozotocin(STZ). And the mice were daily treated by different dose of TFAG (100, 200, 400 mg��kg-1) for 15 d. In order to determine the blood glucose, blood was collected every 3 d. After administration of drug for 16 d, the blood was collected from the eyes and serum insulin and other biochemical indicator were measured. Hematoxylin and eosin stain will be used for histological analysis after collection of pancreatic tissues. Meanwhile, the amount of SOD, CAT and MDA in liver were measured. RESULTS TFAG could significantly inhibit the activity of ��-glucosidase and ��-amylase, and that TFAG had a better inhibitory activity on ��-glucosidase than ��-amylase. The results of in vivo assay showed that TFAG could significantly reduce the levels of blood glucose, LDL, TC and TG, and increase the concentration of insulin and HDL in diabetic mice. HE staining showed that TFAG had some repair effects on pancreatic islet in diabetic mice. It was found that TFAG significantly decreased the levels of MDA, but instead it significantly increased the activities of SOD and CAT in liver of diabetic mice. CONCLUSION The TFAG has significant effect on the hypoglycemic and antioxidant activity, and promote repair of pancreatic islets. Collectively, TFAG are valuable raw material with medicinal and edible properties and high development value.
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