Abstract��OBJECTIVE To study the qualities of protamine sulfate raw material from different species of fish. METHODS The identification and determination of protamine sulfate raw material from different fish species were conducted by HPLC, and the composition of amino acids was tested too. Heavy metals was tested by inductively coupled plasma mass spectrometry. The potency of protamine sulfate was measured by biological assay method. RESULTS At present, the sources of protamine sulfate on the market were mainly salmon and herring. CONCLUSION This HPLC method can be applied to effectively identify and determine protamine sulfate raw material in order to improve its quality control.
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LIU Qian,LI Zhen,ZHANG Yuan,WU Yan-lin, TAN De-jiang, GAO Hua, YANG Hua-xin. Qualities Study of Protamine Sulfate Raw Material from Different Species of Fish. Chinese Pharmaceutical Journal, 2017, 52(16): 1451-1455.
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