A New Iridoid glucoside from the Ripe Fruits of Cornus officinalis Sieb. et Zucc.
QIAO Hao-yi1,2, YE Xian-sheng3, HE Jun4, WANG Xiao-xue4,ZHANG Jia-lin5, PANG Xiao-bin5, ZHANG Wei-ku2,6*, XU Jie-kun1*
1. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China; 2. Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China; 3. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China; 4. Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China; 5. Pharmaceutical Institute, Henan University, Kaifeng 475004, China; 6. Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Beijing 100029, China
Abstract��OBJECTIVE To study the chemical constituents from the ripe fruits of Cornus officinalis Sieb. et Zucc., and evaluate their protection on PC12 cells exposed to oxygen and glucose deprivation.METHODS The compounds were isolated through various chromatographic methods including macroporous adsorption resin, silica gel, ODS, Sephadex LH-20 column chromatography, and preparative HPLC, and their structures were determined through spectroscopic analysis, including 1D and 2D NMR and MS data.RESULTS Eight compounds were isolated from the water extract of the ripe fruits of Cornus officinalis Sieb. et Zucc., and their structures were elucidated as 6��-O-acetyl-7��-O-ethyl morroniside (1), chrysoderol(2), luteolin(3), 5-hydroxymethylfurfural(4), syringate(5),p-hydroxybenzoic acid(6),caffeic acid methyl ester(7), ethyl gallate(8).CONCLUSION Compound 1 is a new iridoid glucoside, and compounds 2, 3, 5, 7 are obtained from Cornus officinalis for the first time. The MTT results show that compound 4 moderately increases the viability of OGD/R treated PC12 cells at the concentration of 1.0 ��mol��L-1.
��尵t��Ҷ��ʤ�� �վ�������ѩ���ż��գ� ������ ��ά�⣬ ������. ɽ������һ���µĻ�ϩ�����������[J]. �й�ҩѧ��־, 2017, 52(14): 1212-1216.
QIAO Hao-yi, YE Xian-sheng, HE Jun, WANG Xiao-xue,ZHANG Jia-lin, PANG Xiao-bin, ZHANG Wei-ku, XU Jie-kun. A New Iridoid glucoside from the Ripe Fruits of Cornus officinalis Sieb. et Zucc.. Chinese Pharmaceutical Journal, 2017, 52(14): 1212-1216.
Flora of China editorial committee. Flora of China (�й�ֲ��־) . Vol 67. Beijing:Science Press, 1990:56-84.
[2]
PANG Y, WANG T S. Summary of the studies of the chemical composition of dogwood fruit . J Nanjing Univ Tradit Chin Med(�Ͼ���ҽҩ��ѧѧ��), 1998, 14(1):61-62.
[3]
ZANG L B, ZHENG Y J. The effect of Cornusofficinalis on antioxidant enzyme activity in mice . Chin J Tradit Med Sci Technol(�й���ҽҩ�Ƽ�), 1994, 1(6):34-35.
[4]
ZANG L B, ZHENG Y J. Study on anti-aging experiment of Cornus officinalis . J Zhejiang Univ Tradit Chin Med(�㽭��ҽѧԺѧ��), 1993,17(5):34.
[5]
Ch.P. 2015(��)(�й�ҩ��2015���һ��) . 2015:27.
[6]
CAO G, ZHANG Y, CONG X D,et al. Research progress on the chemical constituents and pharmacological activities of Fructuscorni. J Chin Pharm Sci(�й�ҩѧӢ�İ�), 2009, 18(3):208-213.
[7]
MA W, WANG K J, CHENG C S, et al. Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy . J Ethnopharmacol, 2014, 153(3):840-845.
[8]
ZHAO L H, DING Y X, ZHANG L, et al. Cornel iridoid glycoside improves memory ability and promotes neuronal survival in fimbria-fornix transected rats . Eur J Pharmacol,2010, 647(1-3):68-74.
[9]
PARK C H, NOH J S, KIM J H, et al. Evaluation of morroniside, iridoid glycoside from corni fructus, on diabetes-induced alterations such as oxidative stress, inflammation,and apoptosis in the liver of type 2 diabetic db/db mices. Biol Pharm Bull, 2011, 34(10):1559-1565. LIN M H, LIU H K, HUANG W J, et al. Evaluation of the potential hypoglycemic and beta-cell protective constituents isolated from corni fructus to tackle insulin-dependent diabetes mellitus . J Agric Food Chem, 2011, 59(14):7743-7751. HU J F, STARKS C M, WILLIAMS R B,et al. Secoiridoid glycosides from the pitcher plant sarraceniaalata. Helv Chim Acta, 2009, 92(2):273-280. HAN S Y, PAN Y, DING G, et al. The application of 1H-NMR and 13C-NMR in identification of iridoid compounds from Cornus officinalis . Chin Arch Tradit Chin Med (��ҽҩѧ��), 2004, 22(1):56-59. TANAKA T,NAKASHIMA T, UEDA T, et al. Facile discrimination of aldose enantiomers by reversed-phase HPLC . Chem Pharm Bull, 2007, 55:899-901. REN F C, WANG L X, WANG F, et al. Chemical constituents from Scilla scilloides . Chin Tradit Herb Drugs (�в�ҩ), 2014, 45(14):1984-1988. XU R L, SHI Y P. Chemical constituents from Artemisiae Anomalae Herba . Chin Tradit Herb Drugs (�в�ҩ), 2014, 45(11):1521-1525. LING X N, ZHOU M Y, SHEN P Q, et al. Chemical constituents from Rehmannia glutinosa . Chin J Chin Mater Med (�й���ҩ��־), 2011, 36(22):3125-3129. LI W, CHEN F K, YIN X W, et al. Chemical constituents of Folium Isatidis . J Shenyang Pharm Univ(����ҩ�ƴ�ѧѧ��), 2005, 20(1):15-16. SHAO J C, HE C H, LI I T, et al. Chemial constituents of Tetrastigma planicauce (Hook. ) Gagnep. Chin Pharm J(�й�ҩѧ��־),2011,46(6):414-417. WANG X S, CHE Q M, LI Y M, et al. A study on chemical constituents in seeds of Crataegus pinnatifida Bge. var. major N. E . Br . Chin J Chin Mater Med (�й���ҩ��־), 1999, 24(12):739-740. ZHAO X H, CHEN D H, SI J Y, et al. Studies on the phenolic acid constituents from Chinese medicine ��sheng-ma��, rhizome of Cimicifuga foetida L . Acta Pharm Sinica(ҩѧѧ��), 2002, 37(7):535-538. HU J, WANG Z Y, KANG M, et al. Chemical constituents of Ardisia faberi . Chin Tradit Herb Drugs(�в�ҩ),2015, 46(14):2048-2051.
2017, Vol. 52 
