LC-MS/MS Method for Quantification of B07 in Rabbit Plasma
SUN Tao1, YIN Lin1, TIAN Fang2, LU Lu3, DONG Ming-xin4, LI Wei-hua2, ZHANG Li-jun1*
1. Shanghai Public Health Clinical Center, Shanghai 201508, China; 2.Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China; 3.Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Basic Medical College, Fudan University, Shanghai 200032, China; 4.Beijing Institute of Biotechnology, Beijing 100071, China
Abstract��OBJECTIVE To devolop a liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the determination of B07 in rabbit plasma.METHODS Analytes were separated from plasma by a combination of alkalinized protein precipitation and liquid-liquid extraction with methyl tert-butyl ether. Chromatographic separation was performed on a Waters Symmetry C18 (4.6 mm��150 mm, 5 ��m)column with the mobile phase consisted of acetonitrile (containing 0.2% acetic acid ) -40 mmol��L-1 ammonium acetate (containing 0.2% acetic acid) in water (80��20). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 529.3��335.4 and m/z 237.1��194.1 for B07 and carbamazepine (internal standard), respectively.RESULTS The method showed a good linearity in a concentration range of 0.31-310 ng��mL-1for B07 was more than 0.99. The intra- and inter-day precision was less than 15% and the absolute recovery was above 75%.CONCLUSION This method was selective and rapid, sensitive for investigating blood drug concentrations in rabbit.
DONG M, LU L, LI H, et al. Design,synthesis, and biological activity of novel 1,4-disubstituted piperidine/piperazine derivatives as CCR5 antagonist-based HIV-1 entry inhibitors[J]. Bioorg Med Chem Letters, 2012,22(9):3284-3286.
[2]
YE J H, ZHOU S M, WANG Q, et al. Design, synthesis and activity evaluation of new anti-HIV-1 CXCR4 inhibitors[J]. Mil Med Sci(����ҽѧ), 2014,38(8):602-607.
[3]
MAO W X, DONG M X, ZHU W G, et al. Design, synthesis and biological activity of bicyclic piperidine-based HIV-1 inhibitors [J]. Chin J Med Chem(�й�ҩ�ﻯѧ��־), 2011,21(3):169-177.
2017, Vol. 52 
