Abstract��OBJECTIVE To investigate the anti-proliferation effects of andrographolide Derivative(ADA) and its potential anti-tumor mechanisms in MCF-7 cells. METHODS The anti-proliferation effects of ADA were assessed by MTT assay in MCF-7 cells. Apoptotic morphological changes of MCF-7 cells after ADA treatment were observed by high content screening (HCS) after AO/EB double staining and DAPI, Apoptotic number was investigated by using flow cytometry. The expression of Bcl-2, Bax,caspase 3,NF-��B p65,p-I��B�� and p-IKK�� were measured using Western blot.RESULTS MTT assay demonstrated that ADA exerted potent anti-tumor effect in a significant concentration-dependent manner;ADA could promote the apoptosis of MCF-7 cells. Western blot results showed that Bcl-2 families and NF-��B p65 and caspase 3 involved in the process of apoptosis, Bcl-2 expression lowered(P<0.05),p-I��B�� and p-IKK�� expression decreased(P<0.01), Bax, NF-��B p65 and caspase 3 expression increased(P<0.01). CONCLUSION ADA could inhibit the proliferation of MCF-7 cells and mechanism may be that it might induce apoptosis by regulated the expression of Bax, Bcl-2 and caspase 3 protein and NF-��B passway.
������, ��ӪӪ, ������, ������, ������, ����. �����������������������ٰ�ϸ��MCF-7������Ӱ�켰�����[J]. �й�ҩѧ��־, 2017, 52(9): 738-742.
GUO Xiao-su, CUI Ying-ying, WU Tian-tian, GUO Ze-lin, SHAO Xiao-ting, HAN Guang. Effect of Andrographolide Derivative ADA on Apoptosis and Its Mechanism of Breast Cancer MCF-7 Cells. Chinese Pharmaceutical Journal, 2017, 52(9): 738-742.
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