Identification of Proteins in Toad Venom by NanoLC-LTQ-Orbitrap Velos Pro
WANG Zi-yue, ZHOU Jing, MA Hong-yue*, ZHU Zhen-hua, QIAN Da-wei, DUAN Jin-ao, WU Qi-nan
Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract��OBJECTIVE To proteins in toad venom. To investigate the by proteomic approach. METHODS The total proteins from the ear-side gland of toad were digested by trypsin, and the peptides were further analyzed by the NanoLC-linear trap quadropole (LTQ)-Orbitrap Velos Pro.. The raw data acquired by mass spectrometer were imported into MaxQuant software for the identification of peptides and proteins. The proteins were categorized based on gene ontology annotation in biological process, cellular component and molecular function. RESULTS A total of 407 protein groups and 880 peptides were identified. There were 76 pathways associated with the identified proteins in toad venom, including the 5-HT receptor mediated signaling pathway, beta adrenergic receptor signaling pathway, blood coagulation, cadherin signaling pathway, cholesterol biosynthesis, etc.. CONCLUSION This study lays the foundation for further exploration of the proteins in toad venom and their functions.
An Editorial Committee of the Administration Bureau of Traditiomal Chinese Medicine. Analytic Chinese Materia Medica(�л�����)[M]. Shanghai:Shanghai Science & Technology Press, 1998:2447-2455.
YIN Y F, ZHANG X L, CHEN J H, et al. Rapid quantification and identification of bioactive components in Venenum Bufonis by HPLC-DAD/ESI-TOF-MS[J]. Chin Pharm J(�й�ҩѧ��־), 2009, 44(8):623-627.
QIAO J J, YUAN Y J . The application of proteomics in medical research[J]. Chin Pharm J(�й�ҩѧ��־), 2003, 38(3):164-167.
HOOD L E, OMENN G S, MORITZ R L, et al. New and improved proteomics technologies for understanding complex biological systems: addressing a grand challenge in the life sciences[J]. Proteomics, 2012, 12(18):2773-2783.
PICKEN M M. Proteomics and mass spectrometry in the diagnosis of renal amyloidosis [J]. Clin Kidney J, 2015, 8(6):665-672.
HUANG C H, KONG Y, DUAN T, et al. Proteomics technology and the research progress on several new targets for drugs[J]. Chin Pharm J(�й�ҩѧ��־), 2005, 40(8):564-568.
XIAO W U, YI-HONG L U, WANG Y X, et al. Research advances in drug-induced liver injury based on systems biology technology[J]. Chin Pharm J(�й�ҩѧ��־), 2014, 49(12):1009-1013.
ZHOU Y Q, LU H. Application of proteomics on toxicity of traditional Chinese medicine injection[J]. Med Inform, 2009, 22(11):2598-2601.
CHEN X, WEN H M, LIU R, et al. Analysis of extracted proteins of scolopendraby nanoflow reversed phase liquid chromatography tandem mass spectectrometry[J]. Chin J Anal Chem(������ѧ), 2014, 42(2): 239-243.
XUAN L, YUE Q X. Technique of proteomics and its application in the research of traditional chinese medicine complex system[J]. Chin J Nat Med(�й���Ȼҩ��), 2009, 7(4):260-269.
MI H, MURUGANJUAN A, CASAGRANDE J T, et al. Large-scale gene function analysis with the PANTHER classification system [J]. Nat Protocols, 2013, 8(8):1551-1566.
PIRMORADIAN M, BUDAMGUNTA H, CHINGIN K, et al. Rapid and deep human proteome analysis by single-dimension shotgun proteomics[J]. Mol Cell Proteomics, 2013, 12(11):3330-3338.
XU M F, DAI J F, XIANG M X, et al. Difference in peptide mass fingerprinting between south buffalo milk casein and soybean protein[J]. J South China Univ Technol(��������ѧѧ��), 2014, 42(6):136-144.
LIU J R, ZHAO X Y, SONG X Q, et al. Study on renaturation, purification and stability of recombinant human Cu-Zn superoxid dismutase[J]. Chin Pharm J(�й�ҩѧ��־), 2007, 42(13):969-974.
HUANG M S, LIU J B, XUAN S U, et al. Effects of spearmint oil on mice with pulmonary fibrosis induced by bleomycin and its impact on the expression of ��-SMA[J]. Chin Pharm J(�й�ҩѧ��־), 2012, 47(7):504-512.
BI S Z, GAO C Z, LI L, et al. Histological, histochemical and ultrastrastructure studies on toad's parotic gland [J]. Nantong Univ Med Sci(��ͨ��ѧѧ��), 1987, 7(4): 1-2.
YE M, GUO D A. Analysis of bufadienolides in the Chinese drug Chansu by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry[J]. Rapid Commun Mass Spectrom, 2005, 19(13):1881-1892.