Exploration into the Anti-Rheumatoid Arthritis Mechanism of Ethyl Acetate Extract from Kadsura longipedunculata Finet et Gagnep
QIN Lang1, CHENG Ya1, MO Dan-dan2, LI Ri-lun1, ZHOU Xiao-lei2, TANG Bing-lan2*
1. Department of Physiology, Guangxi Medical College, Nanning 530023, China; 2. Guangxi Medicinal Plant, Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Nanning 530023, China
Abstract��OBJECTIVE To research the effect and mechanism of ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep.METHODS The 70 SD male rats were randomly divided into 7 groups: normal control group, model group, tripterginum glycosides(TG) group, dexamethasone(DXM) group, ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. low, medium and high dosage groups(0.063, 0.126, 0.252 g��kg-1��d-1), the CIA(collagen induced arthritis) model of rats was adopted. Under 28 days intragastric administration, the rats' state, weight, degree of paw swelling, arthritis index and pathological changes of ankle joints were observed. Their serologic contents of interleukin 1��(IL-1��), interleukin-6(IL-6), and tumor necrosis factor-��(TNF-��) were examined by means of ELISA, and the expression of NF-��B p65 mRNA in joint synovial tissues were tested via quantitative Real-time PCR method.RESULTS The ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. is found obviously effective in inhibiting CIA rats' paw swelling, decreasing serologic content of IL-1��, IL-6, and TNF-��, as well as the expression of NF-��B p65 mRNA in synovial tissues.Its effect is dosage-related, and stronger than that of TG, weaker than that of DXM.CONCLUSIONKadsura longipedunculata Finet et Gagnep. is, to some extent, effective against RA, the mechanism of which is related to the transcription of NF-��B p65.
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2017, Vol. 52 
