Abstract��OBJECTIVE To establish a method for the simultaneous determination of curcumin and germacrone in Curcuma wenyujin by ultrasound-assisted ionic liquid-reversed phase liquid chromatography. METHODS An Agilent ZORBAX Eclipse Plus C18 column (4.6 mm��250 mm, 5 ��m) was used. Gradient elution was conducted with the mobile phase consisting of acetonitrile and 0.1% phosphoric acid at the flow rate of 1.0 mL��min-1. The detection wavelength was set at 265 nm, the flow rate was set at 1.0 mL��min-1, and the column temperature was maintained at 30 ��. RESULTS The result showed that curcumin and germacrone had the highest extraction yield when the [BMIM]PF6 methanol solution concentration of 0.6 mol��L-1 was used as the extraction solvent and the solid-liquid ratio was 1:20. Under the optimized condition, good linearity of curcumin was shown for curcumin in the range of 0.041 2-2.06 ��g(r=0.999 9)and the average recovery was 98.31%. The good linearity of germacrone was shown for germacrone in the range of 0.010 04-0.502 ��g (r=0.999 8)and the average recovery was 97.62%. CONCLUSION This method is convenient, environmentally friendlygreen and highly reproducible. It is beneficial important to study the extraction method of the active gradients of the traditional Chinese medicine Curcuma wenyujin active gradient and its quality control of Curcuma wenyujin.
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2017, Vol. 52 
