Abstract��OBJECTIVE To investigate the therapeutic effects metabolic mechanism of Schisandra chinensis polysaccharide (SCP) on chronic fatigue syndrome (CFS). METHODS CFS rat model was established in a variety of ways such as the bondage, excessive exercise, crowded and noise environment. The Morris water maze test, the open-field test and the tail-suspension test were performed to evaluate the CFS model. The gas chromatography-mass spectrometry (GC-MS) method was conducted to screen the different metabolites in rat urine and analyze the metabolic pathway. RESULTS The body weight of rats were increased and their space exploration and memory ability were strengthened after SCP supplement. The eleven diversity urine metabolites were detected and the involved metabolic pathways were the tricarboxylic acid cycle and the alanine, aspartic and glutamic acid metabolic pathways. CONCLUSION SCP could relieve the chronic fatigue syndrome. The metabolic mechanism is relative to the improvement of SCP on the tricarboxylic acid cycle and the alanine, aspartic and glutamic acid metabolic pathways.
�ذ�ƽ, ����, ���ཪ, ���˱�. ��ζ�Ӷ��Ƕ�����ƣ���ۺ�����������Ч�����л�����о�[J]. �й�ҩѧ��־, 2017, 52(5): 391-397.
CHI Ai-ping, ZHANG Yang, KANG Yi-jiang, YANG Xing-bin. Metabolomics Study on the Treatment of CFS Model Rats with Schisandra chinensis Polysaccharide. Chinese Pharmaceutical Journal, 2017, 52(5): 391-397.
HAO Y. The correlation of chronic fatigue symdrome and its impact factors and TCM constitution and clinical research [D]. Guangzhou:Guangzhou University of Traditional Chinese Medicine, 2013.
WANG B, HONG Y F, WU J X, et al. Clinical features and updated research of chronic fatigue syndrome [J]. Chin J Clin Rehabil (�й��ٴ�����), 2004, 8(35):8078-8081.
WANG Y Y, LI X X, LIU J P, et al. Traditional Chinese medicine for chronic fatigue syndrome:a systematic review of randomized clinical trials [J]. Complement Ther Med, 2014, 22(4):826-833.
LU J H, WU P H, NI J J. Clinical effect of abdominal acupuncture in treatment of chronic fatigue syndrome [J]. Chin J Gen Pract (�л�ȫ��ҽѧ), 2015, 13(4):599-601.
XU K Y, LIN C. Protective effect of polysaccharide from Fructus chinensis on the subcellular level of CCl4 induced liver injury in rats [J]. Chin J Gerontol(�й�����ѧ��־), 2011, 31(8):1352-1354.
ZHAO T, MAO G H, MAO R W, et al. Antitumor and immunomodulatory activity of a water-solube low molecular weight polysaccharide from Schisandra chinensis(Turcz) Baill [J]. Food Chem Toxicol, 2013, 55(3):609-616.
CHI A P, GUO H H, KANG C Z. Extraction of Schisandra chinensis polysaccharide and its influence on glycometabolism of swimming rice in high temperature [J]. Sichuan Sports Sci (�Ĵ�������ѧ), 2014(6):25-27,70.
LIU X H, CHEN Y G, LIN L, et al. Comparison of methods in determination of polysaccharide in Lycium barbarum L [J]. Food Sci (ʳƷ��ѧ), 2009, 34(9):270-272.
BLUMENKRANTZ N, ASBOE-HANSEN G. New method for quantitative determination of uronicacids[J]. Anal Biochem, 1973, 54(2):484-489.
WANG W G, WU Q, HU B K, et al. Comparison of several methods for the determination of protein content in the Polysaccharide of grey tree [J]. Edib Fung Chin(�й�ʳ�þ�), 2003, 22(1):27-30.
LALREMRUTA V, PRASANNA G S. Evaluation of protective effect of Aegle marmelos Corr. in an animal model of chronic fatigue syndrome [J]. Indian J Pharmacol, 2012, 44(3):351-356.
SACHDEVA A K, KUHAD A, CHOPRA K. Epigallocatechin gallate ameliorates behavioral and biochemical deficits in rat model of load-induced chronic fatigue syndrome [J]. Brain Res Bull, 2011, 86(3-4):165-172.
TAKAHASHI T, YU F, ZHU S J, et al. Beneficial effect of brewers' yeast extract on daily activity in a murine model of chronic fatigue syndrome [J]. eCAM, 2006, 3(1):109-115.
CHI A, KANG C, YAN Z, et al. Immunomodulating and antioxidant effects of polysaccharide conjugates from the fruits of ziziphus jujube on chronic fatigue syndrome rats [J]. Carbohydr Polym, 2015, 122:189-196.
NEWSHOLME P, BENDER K, KIELY A, et al. Amino acid metabolism, insulin secretion and diabetes [J]. Biochem Soc Trans, 2007, 35(Pt 5):1180-1186.
ZOU J, YUAN J, LV S, et al. Effects of exercise on behavior and peripheral blood lymphocyte apoptosis in a rat model of chronic fatigue syndrome [J]. J Huazhong Univ Sci-Med (���пƼ���ѧѧ��), 2010, 30(2):258-264.
FERNNDEZ A A, MARTIN L P, MARTINEZ M I, et al. Chronic fatigue syndrome:aetiology, diagnosis and treatment [J]. BMC Psychiatry, 2009, 9(1):1-11.
XING J, YUAN S C, SUN H M, et al. Discrimination of patients with Xiao-Chaihu Tang syndrome using 1H-NMR metabonomics and partial least square analysis [J]. Acta Pharm Sin (ҩѧѧ��), 2015,50(8):1032-1037.
RAO Z L, WANG N, ZHENG J L, et al. Establishment of rat model of chronic fatigue syndrome [J]. Lab Animal Sci (ʵ�鶯���ѧ), 2014, 31(4):24-27.
MASLIAH E, MALLORY M, ALFORD M, et al. Altered expression of synaptic proteins occurs early durig progression of Alzheimer's disease [J]. Neurology, 2001, 56(1):127-129.
YAO P J, ZHU M, PYUN E I, et al. Defects in expression of genes related to synaptic vesicle trafficking in frontal cortex of Alzheimer's disease [J]. Neurol Dis, 2003, 12(2):97-109.