HPLC Fingerprint Analysis and Pattern Recognition of Wild and Cultivated Prunella vulgaris from Different Habitats
PI Sheng-ling1,2,3, HU Yu-zheng1,2,3, PENG Xi1,2,3, LI Ya-mei1,2,3, LIN Li-mei1,2,3*, XIA Bo-hou1,2,3, WU Pin1,2,3*
1. School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410203, China; 2. Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Changsha 410203,China; 3. Collaborative Innovation Center of Resource for Chinese Materia Medica of Hunan Province, Changsha 410203, China
Abstract��OBJECTIVE To develop a method of fingerprint analysis of Prunella vulgaris by HPLC for the quality control of P. vulgaris. METHODS Twenty-eight samples of wild and cultivated P. vulgaris. obtained from different habitats were analyzed. The difference in the chromatographic fingerprints of P. vulgaris.samples between the two varieties was identified by chemometric methods including principal component analysis (PCA) and partial least squares discriminate analysis (PLS-DA). The selected biomarkers were identified by comparing with reference standards. RESULTS The fingerprint of P. vulgaris was established, and 12 common peaks and good similarities were found in the HPLC fingerprints of P. vulgaris from different habitats. PLS-DA result showed obvious distinction between the two varieties of P. vulgaris, while PCA showed poor distinguishing ability. Six compounds were screened as biomarkers, representing major differences between the two varieties. Four of them were identified as rutinum, rosmarinic acid, caffeic acid, and luteolin. CONCLUSION The fingerprint analysis combined with chemical pattern recognition can be applied as a measure for the quality control and comprehensive evaluation of P. vulgaris.
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PI Sheng-ling, HU Yu-zheng, PENG Xi, LI Ya-mei, LIN Li-mei, XIA Bo-hou, WU Pin. HPLC Fingerprint Analysis and Pattern Recognition of Wild and Cultivated Prunella vulgaris from Different Habitats. Chinese Pharmaceutical Journal, 2017, 52(5): 367-371.
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2017, Vol. 52 
