Abstract��OBJECTIVE To study the apoptosis of human gastric cancer cell line BGC-823 induced by liposome of total glucosides from paeonia(TGP liposome ). METHODS The inhibition of cell proliferation was determined by Cell Counting Kit-8 assay.The morphological changes of the apoptosis cells were observed by HE staining,Hoechst 33258 fluorescent staining, transmission electron microscope. Changes of apoptosis related factor Bcl-2 and Bax protein expression were detected by Western blot. RESULTS TGP liposome significantly inhibited proliferation of BGC-823 cells in a dose-dependent manner(P<0.05). GC-823 cells induced by TGP liposome for 48 h was observed on morphological changes such as karyopyknosis and karyorrhexis and apoptotic bodies. Bcl-2 expression was reduced, but Bax expression was increased,so the ratio of Bcl-2 /Bax decreased. CONCLUSION TGP liposome can induce the apoptosis of BGC-823 cells and inhibit its proliferation, its mechanism may be related to down-regulating protein expression of Bcl-2, up-regulating protein expression of Bax, reducing Bcl-2/Bax value.
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BAO Shu-jie, ZHANG Dan, ZHANG Hong, SUN Yu-hong, LIU Ming-hua, QIAO Xi. Apoptosis of Human Gastric Cancer Cell Line BGC-823 Induced by Liposome of Total Glucosides from Paeonia. Chinese Pharmaceutical Journal, 2016, 51(24): 2109-2113.
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2016, Vol. 51 
