目的 建立半枝莲配方颗粒含量测定及HPLC指纹图谱研究方法，为半枝莲配方颗粒的质量评价提供依据。方法 高效液相色谱法，采用Wondasil C₁₈色谱柱(4.6 mm×250 mm，5 μm)，以甲醇-乙腈-0.1%磷酸=12.5∶15∶72.5为流动相，流速1 mL·min^-1，柱温30 ℃，检测波长335 nm测定半枝莲配方颗粒中野黄芩苷和野黄芩素的含量;检测波长320 nm。建立半枝莲药材、水煎剂和配方颗粒的指纹图谱，并进行相关性分析。结果 野黄芩苷和野黄芩素的线性范围分别为0.074 0~0.518 0 μg和0.051 6~0.361 2 μg，平均回收率分别为103.18%、99.99%。建立了半枝莲药材、水煎剂和配方颗粒指纹图谱，半枝莲配方颗粒指纹图谱中的9个共有峰全部可在水煎剂中得到追踪，有8个可在药材中得到追踪。结论 本实验所建立的半枝莲配方颗粒含量测定及指纹图谱方法稳定性、重复性好，可为半枝莲配方颗粒质量控制和评价提供参考。

OBJECTIVE To establish the HPLC methods for determining the contents of the active ingredients and fingerprint chromatogram of Scutellaria barbata formula granules. METHODS The HPLC analysis was carried out on a Wondasil C₁₈ column (4.6 mm×250 mm,5 μm) with mobile phase consisting of methanol-acetonitrile-0.1% phosphoric acid (12.5∶15∶72.5) at the flow rate of 1.0 mL·min^-1. The column temperature was maintained at 30 ℃. The detection wavelength was set at 335 nm to determine the contents of scutellarin and scutellarein and 320 nm to establish the fingerprint chromatogram of Scutellaria barbata formula granules, medical materials, and aqueous decoction. RESULTS The linear ranges of scutellarin and scutellarein were 0.074 0-0.518 0 μg and 0.051 6-0.3612 μg, respectively. The average recoveries were 103.18% and 99.99%, respectively. Nine peaks in the fingerprint chromatogram of formula granules could be tracked in the aqueous decoction, and eight peaks in the fingerprint chromatogram could be tracked in the medical materials. CONCLUSION The methods can provide more information for the quality control of Scutellaria barbata formula granules.