目的 利用响应面法确定蘘荷总黄酮的最佳超声辅助提取工艺，并探讨其抗氧化活性。方法 在单因素实验的基础上，进行4因素3水平的Box-Behnken中心组合实验设计，利用响应面分析优化工艺条件，并对此工艺下的提取液进行抗氧化活性检测。结果 在超声功率300 W时，蘘荷总黄酮最佳提取工艺条件为处理温度60 ℃、乙醇体积分数85.99%、液料比98.08 mL·g^-1、处理时间15 min，此工艺条件下蘘荷总黄酮得率为37.01%，与预测值35.49%的相对误差仅4.28%;1.038 7 mg·mL^-1囊荷总黄酮与56.60 μg·mL^-1Vc的还原力相当，一定浓度的囊荷总黄酮对·OH、DPPH自由基清除率达到73.07%、65.72%，对脂质体过氧化的抑制率达到49.94%。结论 蘘荷总黄酮可作为一种新型抗氧化药物开发，其提取工艺可用响应面法进行回归分析和参数优化。

OBJECTIVE To determine the optimum ultrasonic-assisted extracting process of total flavonoids from Zingiber mioga by response surface methodology, and then study the antioxidant activity of the extract. METHODS On the basis of single factor experiments, a 4-factor, 3-level Box-Behnken center-united experiment was conducted. The Box-Behnken experiment analyzed and optimized the processing conditions by response surface methodology, then the antioxidant activity of the extract was determined. RESULTS The optimum conditions at ultrasonic power of 300 W were as follows treatment temperature 60 ℃, ethanol concentration 85.99%, liquid-to-solid ratio 98.08 mL·g^-1, treatment time 15 min. Under the optimum conditions, the extraction rate of the total flavonoids was 37.01%, with a bias of 4.28% compared with the predicted value of 35.49%; the reducing power of the total flavonoids from Zingiber mioga at the concentration of 1.038 7 mg·mL^-1 was equal to that of vitamin C of 56.60 μg·mL^-1; the ·OH radical scavenging ratio of the total flavonoids from Zingiber mioga was 73.07%, the DPPH radical scavenging ratio was 65.72%, and the inhibition rate of anti-lipid peroxidation was 49.94%. CONCLUSION The total flavonoids from Zingiber mioga can be developed as a new type of antioxidant agents, and regression analysis and parameter optimization of the extracting process can be conducted by using response surface methodology.