Ultrasonic-Assisted Extraction Process of Total Flavonoids from Zingiber mioga and Its Antioxidant Activity
HUANG Ren-shu1,2, QIAN Yan-lin2, LIU Ren-ni2, YU Ting-ting2, YU Xiu-feng2
1. Plant Cell Engineering of Anhui Province Engineering Technology Research Center, Liu��an 237012, China; 2. Faculty of Biological and Pharmaceutical Engineering, West Anhui University, Liu��an 237012, China
Abstract��OBJECTIVE To determine the optimum ultrasonic-assisted extracting process of total flavonoids from Zingiber mioga by response surface methodology, and then study the antioxidant activity of the extract. METHODS On the basis of single factor experiments, a 4-factor, 3-level Box-Behnken center-united experiment was conducted. The Box-Behnken experiment analyzed and optimized the processing conditions by response surface methodology, then the antioxidant activity of the extract was determined. RESULTS The optimum conditions at ultrasonic power of 300 W were as follows treatment temperature 60 ��, ethanol concentration 85.99%, liquid-to-solid ratio 98.08 mL��g-1, treatment time 15 min. Under the optimum conditions, the extraction rate of the total flavonoids was 37.01%, with a bias of 4.28% compared with the predicted value of 35.49%; the reducing power of the total flavonoids from Zingiber mioga at the concentration of 1.038 7 mg��mL-1 was equal to that of vitamin C of 56.60 ��g��mL-1; the ��OH radical scavenging ratio of the total flavonoids from Zingiber mioga was 73.07%, the DPPH radical scavenging ratio was 65.72%, and the inhibition rate of anti-lipid peroxidation was 49.94%. CONCLUSION The total flavonoids from Zingiber mioga can be developed as a new type of antioxidant agents, and regression analysis and parameter optimization of the extracting process can be conducted by using response surface methodology.
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HUANG Ren-shu, QIAN Yan-lin, LIU Ren-ni, YU Ting-ting, YU Xiu-feng. Ultrasonic-Assisted Extraction Process of Total Flavonoids from Zingiber mioga and Its Antioxidant Activity. Chinese Pharmaceutical Journal, 2016, 51(19): 1652-1656.
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2016, Vol. 51 
