OBJECTIVE To develop a method for the determination of iridoid,phenylpropanoid glycosides,and organic acids in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS. METHODS The analysis was carried out on a BDS HYPERSIL C18 column (4.6 mm×250 mm,5 μm) with elution by mobile phase of acetonitrile-water at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 35 ��. The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM) mode. RESULTS Twelve multiple constituents showed good linearity (r>0.999 4) in the range of the tested concentration. The average recoveries of the 12 components were 99.59%-101.24% with relative standard deviations of 0.93%-1.60%. CONCLUSION The established method is accurate and precise,which provides a reliable and effective technique for the quality evaluation of Scrophulariae Radix.
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WANG Sheng-nan,HUA Yu-jiao,XU Li,ZOU Li-si,LIU Xun-hong,LUO Yi-yuan,LIU Juan-xiu,YAN Ying. Simultaneous Determination of Multiple Constituents in Scrophulariae Radix from Different Habitats and Commercial Herbs by UPLC-QTRAP-MS/MS. Chinese Pharmaceutical Journal, 2016, 51(16): 1416-1422.
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2016, Vol. 51 
