Abstract��OBJECTIVE To study the effects of fibroblast growth factor-21(FGF-21) on acute inflammation induced by lipopolysaccharide (LPS)and its mechanism. METHODS Mouse model of acute inflammation was established by injection of LPS and treated with FGF-21 at high, medium and low doses, the pathological changes were detected with HE staining, the expression level of TNF-��, IL-1��, IL-6 and IL-17 in serum and peritoneal macrophage were determined by ELISA and Real-time PCR. NF-��B p65 in macrophage cells was analyzed by confocal laser scanning microscope and Western-blotting. RESULTS FGF-21 treatment reduced lung damage and inflammatory cell infiltration and decreased the expression level of TNF-��, IL-1��, IL-6 and IL-17 in both serum and peritoneal macrophage. The results of confocal laser scanning microscope and Western-blotting both showed that FGF-21 could inhibit NF-��B transferring to the nucleus. CONCLUSION FGF-21 could regulate the immune system by acting on macrophage. Relieving the inflammatory response in mice through NF-��B signal pathway may be one of the mechanisms FGF-21 regulating immune system.
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2016, Vol. 51 
