Extraction and Purification Process of the Active Ingredient Eurycomanone from Eurycoma longifolia Jack
LI Hong-liang1,YU Xiu-ying1,MA Chong-ming2,KIEW Lik-voon3,MOHAMMAD Rais-mustafa3,LI Wei-guo4,TIAN Jing-kui1*
1. College of Biomedical Engineering and Instrument Science�� Zhejiang University�� Hangzhou 310027�� China; 2. Taizhou Institute of Sci. & Tech.�� Nanjing University of Science & Technology�� Taizhou 225300�� China; 3. Department of Pharmacology�� Faculty of Medicine�� University of Malaya�� Kuala Lumpur 50603�� Malaysia; 4. Changshu Qiushi Technology Co.�� Ltd�� Changshu 215500�� China
Abstract��OBJECTIVE To optimize the extraction and purification process of eurycomanone (EN) from Eurycoma longifolia Jack. METHODS Using single factor test and orthogonal test to screen the best conditions of ethanol concentration, extraction time, amount of solvent and extraction times with the index of transfer rate of EN. Resin model was screened by static adsorption and desorption test, purification process of EN was investigated by single factor test and orthogonal test. RESULTS The optimal condition of the extracting was 20-fold water, 2 times with the first 2 and 1 h for the second time. Optimal purification process of HPD100 macroporous resin was 70% amount of saturated adsorption sample, 0.25 g��mL-1 of sample liquid concentration, 3BV��h-1 of sample flow rate, eluting using the 30% ethanol, 3 BV��h-1 of elution velocity. CONCLUSION The optimized process is simple, stable and repeatable, which is suitable for industrial production.
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LI Hong-liang,YU Xiu-ying,MA Chong-ming,KIEW Lik-voon,MOHAMMAD Rais-mustafa,LI Wei-guo,TIAN Jing-kui. Extraction and Purification Process of the Active Ingredient Eurycomanone from Eurycoma longifolia Jack. Chinese Pharmaceutical Journal, 2016, 51(14): 1169-1175.
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2016, Vol. 51 
