不同加工玄参核苷类成分的含量测定及TOPSIS分析

王胜男, 华愉教, 邹立思, 罗益远, 刘训红, 刘娟秀, 严颖, 徐力

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (4) : 274-279.

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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (4) : 274-279. DOI: 10.11669/cpj.2016.04.006
论 著

不同加工玄参核苷类成分的含量测定及TOPSIS分析

  • 王胜男1, 华愉教1, 邹立思1, 罗益远1, 刘训红1*, 刘娟秀1, 严颖1, 徐力2
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Determination and TOPSIS Analysis of Nucleosides and Nucleobases in Differently Processed Scrophulariae Radix Products

  • WANG Sheng-nan1, HUA Yu-jiao1,ZOU Li-si1, LUO Yi-yuan 1, LIU Xun-hong1*, LIU Juan-xiu 1, YAN Ying1, XU Li2
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摘要

目的 建立超高效液相色谱-串联四极杆/线性离子阱质谱(QTRAP-UPLC-MS/MS)同时测定玄参药材中10种核苷类成分含量的方法,分析不同加工方法对玄参核苷类成分含量的影响。方法 采用QTRAP-UPLC-MS/MS技术测定不同加工玄参药材中10种核苷类成分的含量,用TOPSIS法对核苷类成分进行综合评价。结果 玄参核苷类成分中以鸟苷、尿苷、腺苷、尿嘧啶含量较高;不同加工方法对玄参核苷类成分含量具有一定的影响,玄参“发汗”法加工样品中核苷综合质量较好。结论 为探究加工对玄参化学成分的影响及优选玄参适宜产地加工方法提供基础资料。

Abstract

OBJECTIVE To establish a QTRAP-UPLC-MS/MS method for simultaneous determination of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix processed by different methods, and investigate the influences of different processing < br/>METHODS on contents of nucleosides and nucleobases. METHODS The contents of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix processed with different METHODS were simultaneously determined by QTRAP-UPLC-MS/MS, and TOPSIS analysis was used for comprehensive evaluation of nucleosides and nucleobases. RESULTS The contents of guanosine,uridine, adenosine and uracil were relatively high in Scrophulariae Radix.There were differences in contents of nucleosides and nucleobases of Scrophulariae Radix processed with different methods, and the samples processed by method of “sweating” had better quality. CONCLUSION This study provides a scientific basis for investigation on the influence of processing METHODS on contents of nucleosides and nucleobases in Scrophulariae Radixand screening of suitable processing method for Scrophulariae Radix.

关键词

玄参 / 加工方法 / 超高效液相色谱-串联四极杆/线性离子阱质谱 / 核苷 / 含量测定 / TOPSIS分析

Key words

Scrophulariae Radix / processing method / QTRAP-UPLC-MS/MS / nucleoside and nucleobase / simultaneous determination / TOPSIS analysis

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导出引用
王胜男, 华愉教, 邹立思, 罗益远, 刘训红, 刘娟秀, 严颖, 徐力. 不同加工玄参核苷类成分的含量测定及TOPSIS分析[J]. 中国药学杂志, 2016, 51(4): 274-279 https://doi.org/10.11669/cpj.2016.04.006
WANG Sheng-nan, HUA Yu-jiao,ZOU Li-si, LUO Yi-yuan, LIU Xun-hong, LIU Juan-xiu, YAN Ying, XU Li. Determination and TOPSIS Analysis of Nucleosides and Nucleobases in Differently Processed Scrophulariae Radix Products[J]. Chinese Pharmaceutical Journal, 2016, 51(4): 274-279 https://doi.org/10.11669/cpj.2016.04.006
中图分类号: R917   

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基金

江苏省高校优势学科建设工程资助项目(ysxk-2014)
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