目的 建立超高效液相色谱-串联四极杆/线性离子阱质谱(QTRAP-UPLC-MS/MS)同时测定玄参药材中10种核苷类成分含量的方法,分析不同加工方法对玄参核苷类成分含量的影响。方法 采用QTRAP-UPLC-MS/MS技术测定不同加工玄参药材中10种核苷类成分的含量,用TOPSIS法对核苷类成分进行综合评价。结果 玄参核苷类成分中以鸟苷、尿苷、腺苷、尿嘧啶含量较高;不同加工方法对玄参核苷类成分含量具有一定的影响,玄参“发汗”法加工样品中核苷综合质量较好。结论 为探究加工对玄参化学成分的影响及优选玄参适宜产地加工方法提供基础资料。
Abstract
OBJECTIVE To establish a QTRAP-UPLC-MS/MS method for simultaneous determination of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix processed by different methods, and investigate the influences of different processing < br/>METHODS on contents of nucleosides and nucleobases. METHODS The contents of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix processed with different METHODS were simultaneously determined by QTRAP-UPLC-MS/MS, and TOPSIS analysis was used for comprehensive evaluation of nucleosides and nucleobases. RESULTS The contents of guanosine,uridine, adenosine and uracil were relatively high in Scrophulariae Radix.There were differences in contents of nucleosides and nucleobases of Scrophulariae Radix processed with different methods, and the samples processed by method of “sweating” had better quality. CONCLUSION This study provides a scientific basis for investigation on the influence of processing METHODS on contents of nucleosides and nucleobases in Scrophulariae Radixand screening of suitable processing method for Scrophulariae Radix.
关键词
玄参 /
加工方法 /
超高效液相色谱-串联四极杆/线性离子阱质谱 /
核苷 /
含量测定 /
TOPSIS分析
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Key words
Scrophulariae Radix /
processing method /
QTRAP-UPLC-MS/MS /
nucleoside and nucleobase /
simultaneous determination /
TOPSIS analysis
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中图分类号:
R917
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参考文献
[1] Ch. P(2010) . Vol Ⅰ(中国药典2010年版.一部)[S]. 2010: 108.
[2] JI J Y, ZHANG Y, CONG X D, et al. Advances in research of chemcial constituents and quality control of Scrophularia ningpoensi[J]. China Arch Tradit Chin Med (中华中医药学刊), 2010, 28(12): 2507-2510.
[3] HU Y Y, HUANG Z. Advances in research of chemical constituents and pharmacological effects of Scrophularia ningpoensi shemsl[J]. Zhejiang J Tradit Chin Med (浙江中医药大学学报), 2008, 32(2): 268-270.
[4] BAI Y E, YUAN P F, WANG Q H, et al. Determination of harpagide and harpagoside in Scrophulariae Radix by HPLC-UV[J]. China J Chin Mater Med (中国中药杂志), 2011,36(19): 2697-2702.
[5] WANG J H, XIE L H, LIU H Y, et al. HPLC determination of harpagoside and cinnamic acid in xuanshen after processed in different methods[J]. Chin Pharm J(中国药学杂志),2000,35(6):375-378.
[6] HAN L, XU H, LIU X H, et al. Simultaneous determination of six index components in Scrophularianingpoensis by HPCE[J]. Chin Pharm J (中国药学杂志), 2012,47(7): 555-559.
[7] WANG R. Advances in pyrimidine[J]. Lett Bio(生物技术通讯), 2007, 3(3): 539-542.
[8] MA B J, RUAN Y, LIU J K. Chemical study of nucleosides on Cordycepsmilitaris[J]. J Chin Med Mater (中药材), 2007, 30(8): 957-958.
[9] LV A J, WU H. Progress of nucleosides in traditional Chinese medicine[J]. Chin J Info Tradit Chin Med(中国中医药信息杂志), 2006, 13(7): 94-97.
[10] YU J J, CHEN S H, LU G Y. Progress in related pharmacodynamics research on cooling blood and nourishing yin of Radix Scrophulariae[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2007,13(9): 63-66.
[11] DUAN J A, SU S L, LV J L, et al. Traditional experiences and modern cognition on primary processing of traditional Chinese medicinal materials[J]. China J Chin Mater Med (中国中药杂志), 2009, 34(24): 3152-3157.
[12] YAO R Y,WANG F,ZOU Y F, et al. Comprehensive germination quality evaluation of bupleurumchinense seeds of different maturities from qingchuan county with the topsis approach [J]. Bull Bot Res(植物研究), 2014, 34(1) : 108-113.
[13] XIAO X,WANG Y R,MA J, et al. Preparation of enzymecontrolledcolonpositioning tablets of berberinehydrochloride [J]. Beijing J Tradit Chin Med (北京中医药大学学报), 2008,31(12): 847-850.
[14] SUN D M, DONG Y J, XU A L, et al. Rapid test of twele flavonoid compounds in Yinxing linzhi Capsules by UPLC-Q-TOF-MS[J]. Chin Tradit Pat Med (中成药), 2015, 37(2) : 320-324.
[15] DUN Z C,ZHU G H,ZOU Z Q,et al. Determination of osthole and imperatorin in Radix Angelicae Pubescentis from different habitats by HPLC-MS/MS[J]. Tradit Chin Drug Res Clin Pharm(中药新药与临床药理), 2015, 26(1) : 105-108.
[16] LU Y N, TAN G G. Analysis on chemical constituents of Xiongguidecotion by HPLC-TOF-MS[J]. J Pharm Prac(药学实践杂志), 2015, 33(1) : 49-71.
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脚注
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基金
江苏省高校优势学科建设工程资助项目(ysxk-2014)
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