Determination of ASC-J9 in Rat Blood by HPLC-MS/MS
WANG Feng-fei1, ZHANG Xiao-qian2, CAO Cong3, SHENTU Jian-zhong4*,TANG Lan1*
1.Zhejiang University of Technology�� Hangzhou 310014��China; 2. Wenzhou Medical University��Wenzhou 325035��China; 3.Zhejiang University�� Hangzhou 310023��China; 4. The First Affiliated Hospital of Zhejiang University�� Hangzhou 310001��China
Abstract��OBJECTIVE To establish an HPLC-MS/MS method for determination of ASC-J9 in rat blood. METHODS After liquid-liquid extraction, ASC-J9 was separated on a Symmetry C18colume, with mobile phase of acetonitrile-water containing 0.1% formic acid and 10 mmol��L-1 ammonium formate.The flow rate was 1.0 mL��min-1, mass shunt was 0.4 mL��min-1, and column temperature was main tained at 35 ��. Quantification was performed in positive ion multiple-reaction-monitoring(MRM) mode. RESULTS The calibration curve of ASC-J9 had good linearity in the concentration range of 3.54-1 180 ng��mL-1. The extraction recovery rate was within 83.19% to 87.27%,and the intra-day and inter-day RSDs were both less than 8.89%. CONCLUSION This method is specific, sensitive and suitable for determination of ASC-J9 in rat blood.
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WANG Feng-fei, ZHANG Xiao-qian, CAO Cong, SHENTU Jian-zhong,TANG Lan. Determination of ASC-J9 in Rat Blood by HPLC-MS/MS. Chinese Pharmaceutical Journal, 2015, 50(22): 1996-1999.
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