目的 探讨姜黄素对哮喘气道重塑大鼠骨桥蛋白(OPN)及mRNA表达的影响及其在哮喘气道重塑中的作用。方法 将24只SD大鼠分为3组,正常对照组(N组)、哮喘组(A组)和姜黄素组(C组),每组8只。卵清蛋白(OVA)致敏和激发制备哮喘气道重塑模型。图像分析技术测量大鼠支气管壁厚度(Wat)和平滑肌厚度(Wam),免疫组化法和逆转录-聚合酶链反应(RT-PCR)法测定大鼠肺组织骨桥蛋白的蛋白及mRNA表达,对支气管壁厚度、平滑肌厚度与骨桥蛋白蛋白和mRNA的表达进行相关性分析。结果 哮喘组支气管壁厚度和平滑肌厚度均高于对照组。与哮喘组相比,姜黄素组支气管壁厚度和平滑肌厚度显著下降。免疫组化检测提示,哮喘组骨桥蛋白蛋白表达显著高于正常对照组,姜黄素组相对哮喘组显著下降,差异具有统计学意义(P<0.01)。逆转录-聚合酶链反应法检测显示,哮喘组骨桥蛋白mRNA表达显著高于正常对照组,姜黄素组相对哮喘组显著下降,差异具有统计学意义(P<0.01)。肺组织中支气管壁厚度、平滑肌厚度与骨桥蛋白的蛋白、mRNA表达均呈正相关。结论 姜黄素抑制哮喘气道重塑,可能通过下调大鼠骨桥蛋白的表达发挥作用。
Abstract
OBJECTIVE To observe the effects of curcumin on the protein and mRNA expression of osteopontin(OPN) in asthmatic airway remodeling rats. METHODS Twenty-four SD rats were randomly divided into 3 groups:normal control group(N group),asthma group(A group) and curcumin group (C group),with each 8 rats.The asthmatic airway remodeling model was established by sensitization and challenge with ovalbumin(OVA). The total brochial wall thickness(Wat) and the brochial smooth muscle thickness(Wam) were measured by image analysis system. The protein expression of OPN in lung homogenats were detected by immunohistochemistry (IHC) and the mRNA expression measured by reverse transcription-polymerase chain reaction(RT-PCR). Besides,correlative analysis between Wam/Wat and the expression of OPN was studied. RESULTS Wat and Wam of asthma group were significant higher than those of normal control group.Compared with asthma group, those of curcumin group were significant lower(P<0.01, respectively). The protein expression of OPN detected by IHC in asthma group were significant higher than those in normal control group.Compared with asthma group, those in curcumin group were significant lower(P<0.01, respectively).The mRNA expression of OPN detected by RT-PCR in asthma group were significant higher than those of normal control group.Compared with asthma group, those in curcumin group were significant lower(P<0.01,respectively). There was significantly positive correlation between Wat and the protein/mRNA expression of OPN,also between Wam and the protein/mRNA expression of OPN.CONCLUSION Curcumin may inhibit asthmatic rat airway remodeling partly through the down regulation of OPN.
关键词
大鼠 /
哮喘 /
气道重塑 /
骨桥蛋白 /
姜黄素
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Key words
:rat /
asthma /
airway remodeling /
osteopontin /
curcumin
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中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金资助项目(30973232;81100015);温州市科技局资助项目(Y20140050)
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