Effect and Mechanism of Apple Polysaccharides on Experimental Colitis Associated Colorectal Cancer in Mice
ZHANG Dian1a,2,WANG Fen-xia3, MI Man1b, SUN Yang 2 ,LI Yu-hua2, FAN Lei2, LIU Li1, MEI Qi-bing2*
1.a.Department of Pathogen Biology and Immunology; b.Department of Pharmacology,Xi��an Medical University, Xi��an 710021,China; 2. Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi��an 710032, China; 3. Department of Pharmacy, Shaanxi Friendship Hospital,Xi��an 710068,China
Abstract��OBJECTIVE To study the effects and mechanisms of apple polysaccharides(AP) on tumorigenesis in a mouse model of colitis-associated colon cancer. METHODS AP was obtained from apple pomace and its protective efficacy was evaluated on carcinogenesis in a mouse model of colitis-associated colon cancer induced by azoxymethane (AOM) and dextran sodium sulfate (DSS). The effects of AP on TLR4/MyD88/NF-��B pathway were measured using immunohistochemistry , ELISA and Western blot.The serum were collected and TNF-�� was measured by ELISA kits. RESULTS After 20 weeks of continuous treatment, the incidence of colon cancer formation was 95% in the mice treated with AOM/DSS (model group), and these reduced to 26%, 10% and 5% in AP (1.25%, 2.5% and 5%) treatment group respectively. Western blot analysis demonstrated that TLR4 (membrane protein), MyD88, NF-��B p65 (nuclear protein) expression decreased significantly at protein level; and the secretion of TNF-�� decreased in control group(P<0.05). CONCLUSION AP could protect ICR mice from CACC effectively and the possible mechanism may be related to the inhibition of TLR4/MyD88/ NF-��B pathways.
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ZHANG Dian,WANG Fen-xia, MI Man, SUN Yang ,LI Yu-hua, FAN Lei, LIU Li, MEI Qi-bing. Effect and Mechanism of Apple Polysaccharides on Experimental Colitis Associated Colorectal Cancer in Mice. Chinese Pharmaceutical Journal, 2015, 50(17): 1527-1531.
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2015, Vol. 50 
