Identification of Geranii Herbae Using ITS2 Sequence
HE Hai-bo1,2, XIONG Chao1,2, GUO Li-cheng2,3, TU Yuan1,2, CHU Yang1, WANG Bo4, LI Dan-ping4,SUN Wei1*, HU Zhi-gang1,2*
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Beijing 100700, China; 2. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China; 3. Guangxi Institude of Minority Medicine, Nanning 530001, China; 4. Hubei Institute for Food and Drug Control, Wuhan 430064, China
Abstract��To provide the molecular basis for standard medicine market management, a new method to identify multi-origin traditional Chinese medicine Geranii herba was established by ITS2 sequence analysis. METHODS As a prelude to species identification, Erodium stephaniahum willd., Geranium carolinianum L., Geranium wilfordii Maxim., together with their relatives adulterants, were sampled. A reference ITS2 sequence library was developed, including 82 ITS2 sequences of 28 species download from GenBank. Based on the intra-species, inter-species divergence, variable sites and the neighbor-joining (NJ) phylogenetic tree observed, a molecular identification method was established to the multi-origin herbs Geranium using ITS2 sequence. RESULTS The NJ trees showed three origin species formed one monophyletic clade respectively; in addition any one of multi-origin herbs Geranium and its relatives adulterants could be distinguished clearly. Using this method, 18 random copies of Geranium herb collected from market were analyzed, 16 of which are genuine, 2 as adulterants. CONCLUSION Therefore ITS2 sequence is suitable to be as a barcode to identify multi-origin herbs Geranium and their relatives adulterants.
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HE Hai-bo, XIONG Chao, GUO Li-cheng, TU Yuan, CHU Yang, WANG Bo, LI Dan-ping,SUN Wei, HU Zhi-gang. Identification of Geranii Herbae Using ITS2 Sequence. Chinese Pharmaceutical Journal, 2015, 50(17): 1505-1511.
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2015, Vol. 50 
