目的 基于叶绿体psbA-trnH序列对肉桂类药材进行鉴定研究,促进规范用药,保障食品安全规范。方法 对8种肉桂类药材psbA-trnH序列进行扩增和测序,应用MEGA 5.0分析其种内种间K2P遗传距离,并构建系统进化树。结果 肉桂psbA-trnH序列长度368 bp,种内序列高度保守,肉桂与其近缘物种种间变异位点多,种间K2P遗传距离明显大于种内K2P遗传距离,NJ树显示肉桂能与近缘物种完全区分开。结论 基于psbA-trnH序列的DNA条形码技术能够准确鉴定肉桂及其相关近缘种。
Abstract
OBJECTIVE To introduce DNA barcoding based on plastid intergenic region psbA-trnH to authenticate medicinal and edible materials Cinnamon cassia to promote the standard practice of medication and foods. METHODS The genomic DNA of eight Cinnamomum species were extracted successfully. The Kimura 2-parameter (K2P) distances were calculated and phylogenetic tree was constructed using software MEGA 5.0. RESULTS The sequence length of the psbA-trnH of Cinnamomum cassia was 368 bp. The psbA-trnH intra-specific genetic distance of C. cassia was highly conservative,which was shorter than the interspecific ones. The NJ tree indicated that Cinnamomi Cortex could be distinguished from its adulterants obviously. CONCLUSION The psbA-trnH intergenic spacer can be used to distinguish Cinnamomi Cortex from its adulterants accurately
关键词
肉桂 /
樟属 /
psbA-trnH /
鉴定 /
DNA条形码
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Key words
Cinnamomi Cortex /
Cinnamomum /
psbA-trnH /
authentication /
DNA barcoding
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中图分类号：
R282
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脚注
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基金
国家科技支撑计划(2011BAI07B08);广西壮族自治区卫生厅中医药科技专项(GZZY13-39)
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